BMRB Entry 51483
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51483
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Title: Solid-state NMR assignment of fibrils formed by tau(297-391) PubMed: 36570831
Deposition date: 2022-06-08 Original release date: 2023-01-30
Authors: Siemer, Ansgar; Serpell, Louise
Citation: Al-Hilaly, Youssra; Hurd, Connor; Rickard, Janet; Harrington, Charles; Storey, John; Wischik, Claude; Serpell, Louise; Siemer, Ansgar. "Solid-state NMR of paired helical filaments formed by the core tau fragment tau(297-391)" Front. Neurosci. 16, 988074-988074 (2022).
Assembly members:
entity_1, polymer, 95 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pRK172
Entity Sequences (FASTA):
entity_1: IKHVPGGGSVQIVYKPVDLS
KVTSKCGSLGNIHHKPGGGQ
VEVKSEKLDFKDRVQSKIGS
LDNITHVPGGGNKKIETHKL
TFRENAKAKTDHGAE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 160 |
| 15N chemical shifts | 36 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | tau(297-391) | 1 |
Entities:
Entity 1, tau(297-391) 95 residues - Formula weight is not available
| 1 | ILE | LYS | HIS | VAL | PRO | GLY | GLY | GLY | SER | VAL | ||||
| 2 | GLN | ILE | VAL | TYR | LYS | PRO | VAL | ASP | LEU | SER | ||||
| 3 | LYS | VAL | THR | SER | LYS | CYS | GLY | SER | LEU | GLY | ||||
| 4 | ASN | ILE | HIS | HIS | LYS | PRO | GLY | GLY | GLY | GLN | ||||
| 5 | VAL | GLU | VAL | LYS | SER | GLU | LYS | LEU | ASP | PHE | ||||
| 6 | LYS | ASP | ARG | VAL | GLN | SER | LYS | ILE | GLY | SER | ||||
| 7 | LEU | ASP | ASN | ILE | THR | HIS | VAL | PRO | GLY | GLY | ||||
| 8 | GLY | ASN | LYS | LYS | ILE | GLU | THR | HIS | LYS | LEU | ||||
| 9 | THR | PHE | ARG | GLU | ASN | ALA | LYS | ALA | LYS | THR | ||||
| 10 | ASP | HIS | GLY | ALA | GLU |
Samples:
sample_1: tau(297-391), [U-100% 13C; U-100% 15N], 300 uM; H2O 100%; sodium phosphate 10 mM; DTT 10 mM
sample_2: tau(297-391), [U-100% 13C; U-100% 15N], 300 uM; H2O 100%; sodium phosphate 10 mM
sample_conditions_1: ionic strength: 0 M; pH: 7.0; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 13C-13C DREAM | sample_1 | isotropic | sample_conditions_1 |
| 2D 15N-13C NCA | sample_1 | isotropic | sample_conditions_1 |
| 1D 13C RINEPT | sample_1 | isotropic | sample_conditions_1 |
| 1D 13C CP | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C INEPT HETCOR | sample_1 | isotropic | sample_conditions_1 |
| 2D 15N-13C NCO | sample_1 | isotropic | sample_conditions_1 |
| 3D NCOCA | sample_1 | isotropic | sample_conditions_1 |
| 3D NCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D NCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D CANcoCA | sample_1 | isotropic | sample_conditions_1 |
| 2D 13C-13C PARIS | sample_1 | isotropic | sample_conditions_1 |
| 1D 13C CP | sample_2 | isotropic | sample_conditions_1 |
| 1D 13C RINEPT | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-13C INEPT HETCOR | sample_2 | isotropic | sample_conditions_1 |
Software:
VNMRj v4.0 - collection
NMRPipe v9.4 - processing
CARA v1.9.1.7 - data analysis
NMR spectrometers:
- Agilent DD2 600 MHz