BMRB Entry 51506

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51506

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Title:
Solid-state NMR 1H-13C-15N assignment of African cichlid nackednavirus capsid protein
Deposition date:
2022-06-29
Original release date:
2023-06-30
Authors:
Pfister, Sara; Meier, Beat; Wiegand, Thomas; Bockmann, Anja; Malar, Alexander
Citation:

Citation: Pfister, Sara; Rabl, Julius; Wiegand, Thomas; Mattei, Simone; Malar, Alexander; Lecoq, Lauriane; Seitz, Stefan; Bartenschlager, Ralf; Bockmann, Anja; Nassal, Michael; Boehringer, Daniel; Meier, Beat. "Structural conservation of HBV-like capsid proteins over hundreds of millions of years despite the shift from non-enveloped to enveloped life-style"  Nat. Commun. 14, 1574-1574 (2023).
PubMed: 36949039

Assembly members:

Assembly members:
entity_1, polymer, 175 residues, 3567274 Da.

Natural source:

Natural source:   Common Name: bony fishes   Taxonomy ID: 27755   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Ophthalmotilapia ventralis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRSF_T7-CNDVcOpt

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGTFIELVKNMKGYKELLLP MEMVPLPAVVLKHVKLILTS QKEHQPWMTEMALKADQCLI HKATLDLAGKATSNEAKPLI EAMQQIILAMTRELWGQIQR HHYGIVQVEHYVKQITLWQD TPQAFRGDQPKPPSFRSDGP TRGQGSFRPFFRGRGRGRGR GRGSQSPARKGPLPK

Data sets:
Data typeCount
13C chemical shifts443
15N chemical shifts108
1H chemical shifts172

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1African cichlid nackednavirus capsid1

Entities:

Entity 1, African cichlid nackednavirus capsid 175 residues - 3567274 Da.

1   METGLYTHRPHEILEGLULEUVALLYSASN
2   METLYSGLYTYRLYSGLULEULEULEUPRO
3   METGLUMETVALPROLEUPROALAVALVAL
4   LEULYSHISVALLYSLEUILELEUTHRSER
5   GLNLYSGLUHISGLNPROTRPMETTHRGLU
6   METALALEULYSALAASPGLNCYSLEUILE
7   HISLYSALATHRLEUASPLEUALAGLYLYS
8   ALATHRSERASNGLUALALYSPROLEUILE
9   GLUALAMETGLNGLNILEILELEUALAMET
10   THRARGGLULEUTRPGLYGLNILEGLNARG
11   HISHISTYRGLYILEVALGLNVALGLUHIS
12   TYRVALLYSGLNILETHRLEUTRPGLNASP
13   THRPROGLNALAPHEARGGLYASPGLNPRO
14   LYSPROPROSERPHEARGSERASPGLYPRO
15   THRARGGLYGLNGLYSERPHEARGPROPHE
16   PHEARGGLYARGGLYARGGLYARGGLYARG
17   GLYARGGLYSERGLNSERPROALAARGLYS
18   GLYPROLEUPROLYS

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks