BMRB Entry 51535
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51535
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Title: Backbone assignment for Functional Amyloid in pseudomonas FapA PubMed: 36368411
Deposition date: 2022-07-28 Original release date: 2023-06-30
Authors: Shin, Ben; Kumar, Amit; Xu, Yingqi; Matthews, Steve
Citation: Ramussen, Helena; Kumar, Amit; Shin, Ben; Stylianou, Fisentzos; Sewell, Lee; Xu, Yingqi; Otzen, Daniel; Pedersen, Jan; Matthews, Steve. "FapA is an Intrinsically Disordered Chaperone for Pseudomonas Functional Amyloid FapC" J. Mol. Biol. 435, 167878-167878 (2023).
Assembly members:
entity_1, polymer, 147 residues, Formula weight is not available
Natural source: Common Name: Pseudomonas putida Taxonomy ID: 303 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas putida
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pRSF1b
Entity Sequences (FASTA):
entity_1: MGHHHHHHHDYDIPTTENLY
FQGEDGIIVTGRNVQGFMVG
RPSFGKDPYPSTANANPSQQ
ILRATGGELSDNDFAGVSSG
STITRAILPNGDLPGLSNTL
GNNSAGLGAGAAAGHASGNS
LGGQISGSIERGLAPLNNIG
AMMGVQP
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 136 |
15N chemical shifts | 90 |
1H chemical shifts | 90 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | FapA | 1 |
Entities:
Entity 1, FapA 147 residues - Formula weight is not available
1 | MET | GLY | HIS | HIS | HIS | HIS | HIS | HIS | HIS | ASP | ||||
2 | TYR | ASP | ILE | PRO | THR | THR | GLU | ASN | LEU | TYR | ||||
3 | PHE | GLN | GLY | GLU | ASP | GLY | ILE | ILE | VAL | THR | ||||
4 | GLY | ARG | ASN | VAL | GLN | GLY | PHE | MET | VAL | GLY | ||||
5 | ARG | PRO | SER | PHE | GLY | LYS | ASP | PRO | TYR | PRO | ||||
6 | SER | THR | ALA | ASN | ALA | ASN | PRO | SER | GLN | GLN | ||||
7 | ILE | LEU | ARG | ALA | THR | GLY | GLY | GLU | LEU | SER | ||||
8 | ASP | ASN | ASP | PHE | ALA | GLY | VAL | SER | SER | GLY | ||||
9 | SER | THR | ILE | THR | ARG | ALA | ILE | LEU | PRO | ASN | ||||
10 | GLY | ASP | LEU | PRO | GLY | LEU | SER | ASN | THR | LEU | ||||
11 | GLY | ASN | ASN | SER | ALA | GLY | LEU | GLY | ALA | GLY | ||||
12 | ALA | ALA | ALA | GLY | HIS | ALA | SER | GLY | ASN | SER | ||||
13 | LEU | GLY | GLY | GLN | ILE | SER | GLY | SER | ILE | GLU | ||||
14 | ARG | GLY | LEU | ALA | PRO | LEU | ASN | ASN | ILE | GLY | ||||
15 | ALA | MET | MET | GLY | VAL | GLN | PRO |
Samples:
sample_1: FapA, [U-98% 13C; U-98% 15N], 0.2 mM; sodium phosphate 20 mM
sample_conditions_1: ionic strength: 0.02 M; pH: 6.5; pressure: 1 atm; temperature: 283 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 950 MHz
- Bruker AVANCE III 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts