BMRB Entry 51538
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51538
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Title: Backbone assignment for Functional Amyloid in pseudomonas FapB PubMed: 36368411
Deposition date: 2022-08-01 Original release date: 2023-06-30
Authors: Sewell, Lee; Stylianou, Fisentzos; Xu, Yingqi; Matthews, Steve
Citation: Rasmussen, Helena; Kumar, Amit; Shin, Ben; Stylianou, Fisentzos; Sewell, Lee; Xu, Yingqi; Otzen, Daniel; Pedersen, Jan; Matthews, Steve. "FapA is an Intrinsically Disordered Chaperone for Pseudomonas Functional Amyloid FapC" J. Mol. Biol. 435, 167878-167878 (2023).
Assembly members:
entity_1, polymer, 174 residues, Formula weight is not available
Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
entity_1: MSHHHHHHGTDSNNQALIDN
AGKQYTGVLSVNQAAGNQHQ
QINSRAISLGGQASDALIQK
LDGKVDPSLNASAAIQGSAF
SNGNGILGVNQSAGANNQMI
NAVRISTGPQSVDDNVLSQQ
NMTLLPDSRSPSTTGSRQVV
TSDQAFTGSRGVVQVNQSAG
VGNRMANTLGVTIK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 293 |
| 15N chemical shifts | 158 |
| 1H chemical shifts | 158 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FapB | 1 |
Entities:
Entity 1, FapB 174 residues - Formula weight is not available
| 1 | MET | SER | HIS | HIS | HIS | HIS | HIS | HIS | GLY | THR | ||||
| 2 | ASP | SER | ASN | ASN | GLN | ALA | LEU | ILE | ASP | ASN | ||||
| 3 | ALA | GLY | LYS | GLN | TYR | THR | GLY | VAL | LEU | SER | ||||
| 4 | VAL | ASN | GLN | ALA | ALA | GLY | ASN | GLN | HIS | GLN | ||||
| 5 | GLN | ILE | ASN | SER | ARG | ALA | ILE | SER | LEU | GLY | ||||
| 6 | GLY | GLN | ALA | SER | ASP | ALA | LEU | ILE | GLN | LYS | ||||
| 7 | LEU | ASP | GLY | LYS | VAL | ASP | PRO | SER | LEU | ASN | ||||
| 8 | ALA | SER | ALA | ALA | ILE | GLN | GLY | SER | ALA | PHE | ||||
| 9 | SER | ASN | GLY | ASN | GLY | ILE | LEU | GLY | VAL | ASN | ||||
| 10 | GLN | SER | ALA | GLY | ALA | ASN | ASN | GLN | MET | ILE | ||||
| 11 | ASN | ALA | VAL | ARG | ILE | SER | THR | GLY | PRO | GLN | ||||
| 12 | SER | VAL | ASP | ASP | ASN | VAL | LEU | SER | GLN | GLN | ||||
| 13 | ASN | MET | THR | LEU | LEU | PRO | ASP | SER | ARG | SER | ||||
| 14 | PRO | SER | THR | THR | GLY | SER | ARG | GLN | VAL | VAL | ||||
| 15 | THR | SER | ASP | GLN | ALA | PHE | THR | GLY | SER | ARG | ||||
| 16 | GLY | VAL | VAL | GLN | VAL | ASN | GLN | SER | ALA | GLY | ||||
| 17 | VAL | GLY | ASN | ARG | MET | ALA | ASN | THR | LEU | GLY | ||||
| 18 | VAL | THR | ILE | LYS |
Samples:
sample_1: FapB, [U-98% 13C; U-98% 15N], 0.2 mM
sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 950 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts