BMRB Entry 51539

Name: Backbone assignment for Functional Amyloid in pseudomonas FapC
Published: 2023-06-30

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51539

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Backbone assignment for Functional Amyloid in pseudomonas FapC PubMed: 36368411

Deposition date: 2022-08-01 Original release date: 2023-06-30

Authors: Sewell, Lee; Stylianou, Fisentzos; Xu, Yingqi; Matthews, Steve

Citation: Rasmussen, Helena; Kumar, Amit; Shin, Ben; Stylianou, Fisentzos; Sewell, Lee; Xu, Yingqi; Otzen, Daniel; Pedersen, Jan; Matthews, Steve. "FapA is an Intrinsically Disordered Chaperone for Pseudomonas Functional Amyloid FapC" J. Mol. Biol. 435, 167878-167878 (2023).

Assembly members:
entity_1, polymer, 235 residues, Formula weight is not available

Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):
entity_1: MGPAEKWKPTPAPTGTVAAA VTDTQVSKDNKFDDTKTLNN AGANGSLSNSKGNLGANIAV GSGNQQDNAAAITSSAGDAA TVFAVADIYQQSKDNKFTNK GTQNNALLNNSANNSSGNVG VNVAAGQGNQQKNNLAIVTA NGKNVAAASNTEQVSLDNHF LNEASSKHSYKPQYVVNNAG LLNSANNASGNIGVNVAAGA GNQQSNTLTLGSGCTVCAAG TGSKLAFLEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	394
15N chemical shifts	215
1H chemical shifts	215

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FapC	1

Entities:

Entity 1, FapC 235 residues - Formula weight is not available

1

MET

GLY

PRO

ALA

GLU

LYS

TRP

LYS

PRO

THR

2

PRO

ALA

PRO

THR

GLY

THR

VAL

ALA

3

VAL

THR

ASP

THR

GLN

VAL

SER

LYS

ASP

ASN

4

LYS

PHE

ASP

THR

LYS

THR

LEU

ASN

5

ALA

GLY

ALA

ASN

GLY

SER

LEU

SER

ASN

SER

6

LYS

GLY

ASN

LEU

GLY

ALA

ASN

ILE

ALA

VAL

7

GLY

SER

GLY

ASN

GLN

ASP

ASN

ALA

8

ALA

ILE

THR

SER

ALA

GLY

ASP

ALA

9

THR

VAL

PHE

ALA

VAL

ALA

ASP

ILE

TYR

GLN

10

GLN

SER

LYS

ASP

ASN

LYS

PHE

THR

ASN

LYS

11

GLY

THR

GLN

ASN

ALA

LEU

ASN

12

SER

ALA

ASN

SER

GLY

ASN

VAL

GLY

13

VAL

ASN

VAL

ALA

GLY

GLN

GLY

ASN

GLN

14

GLN

LYS

ASN

LEU

ALA

ILE

VAL

THR

ALA

15

ASN

GLY

LYS

ASN

VAL

ALA

SER

ASN

16

THR

GLU

GLN

VAL

SER

LEU

ASP

ASN

HIS

PHE

17

LEU

ASN

GLU

ALA

SER

LYS

HIS

SER

TYR

18

LYS

PRO

GLN

TYR

VAL

ASN

ALA

GLY

19

LEU

ASN

SER

ALA

ASN

ALA

SER

GLY

20

ASN

ILE

GLY

VAL

ASN

VAL

ALA

GLY

ALA

21

GLY

ASN

GLN

SER

ASN

THR

LEU

THR

LEU

22

GLY

SER

GLY

CYS

THR

VAL

CYS

ALA

GLY

23

THR

GLY

SER

LYS

LEU

ALA

PHE

LEU

GLU

HIS

24

HIS

Samples:

sample_1: FapC, [U-98% 13C; U-98% 15N], 0.2 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CACB	sample_1	isotropic	sample_conditions_1

Software:

SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 800 MHz
Bruker AVANCE III 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts