BMRB Entry 51624
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51624
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: CPSF73-CPSF100 C-terminal heterodimer PubMed: 36723825
Deposition date: 2022-09-12 Original release date: 2023-02-08
Authors: Thore, Stephane; Mackereth, Cameron
Citation: Thore, Stephane; Fribourg, Sebastien; Mackereth, Cameron. "1H, 15N and 13C resonance assignments of a minimal CPSF73-CPSF100 C-terminal heterodimer" Biomol. NMR Assignments 17, 43-48 (2023).
Assembly members:
entity_1, polymer, 119 residues, Formula weight is not available
entity_2, polymer, 116 residues, Formula weight is not available
Natural source: Common Name: Encephalitozoon cuniculi Taxonomy ID: 6035 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Encephalitozoon cuniculi
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pMCN
Entity Sequences (FASTA):
entity_1: GHMLAKDNEAKIFTGVEGEF
EGIIIGTEDDIRIYKRDELI
ESKYKEMCIYERQRIPYNST
AVLLKQVLVDGFEELVEVES
GFMVGNVHIVLSGQEVVLEW
KSSYTDDVLAISISKMISD
entity_2: MSDVSMGMVKLDKGFDALNY
RAIGTDSVASFRGVRDGDMV
RCIGEGPRMVIGHADINEMR
RMIVEGSMRVEQEENGLLVE
DCVWIRVSGDGVTIDGRDSG
VFYAVRDVVYRSSAFI
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 889 |
| 15N chemical shifts | 230 |
| 1H chemical shifts | 1572 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CPSF73 CTD1-CTD2 | 1 |
| 2 | CPSF100 CTD1-CTD2 | 2 |
Entities:
Entity 1, CPSF73 CTD1-CTD2 119 residues - Formula weight is not available
| 1 | GLY | HIS | MET | LEU | ALA | LYS | ASP | ASN | GLU | ALA | ||||
| 2 | LYS | ILE | PHE | THR | GLY | VAL | GLU | GLY | GLU | PHE | ||||
| 3 | GLU | GLY | ILE | ILE | ILE | GLY | THR | GLU | ASP | ASP | ||||
| 4 | ILE | ARG | ILE | TYR | LYS | ARG | ASP | GLU | LEU | ILE | ||||
| 5 | GLU | SER | LYS | TYR | LYS | GLU | MET | CYS | ILE | TYR | ||||
| 6 | GLU | ARG | GLN | ARG | ILE | PRO | TYR | ASN | SER | THR | ||||
| 7 | ALA | VAL | LEU | LEU | LYS | GLN | VAL | LEU | VAL | ASP | ||||
| 8 | GLY | PHE | GLU | GLU | LEU | VAL | GLU | VAL | GLU | SER | ||||
| 9 | GLY | PHE | MET | VAL | GLY | ASN | VAL | HIS | ILE | VAL | ||||
| 10 | LEU | SER | GLY | GLN | GLU | VAL | VAL | LEU | GLU | TRP | ||||
| 11 | LYS | SER | SER | TYR | THR | ASP | ASP | VAL | LEU | ALA | ||||
| 12 | ILE | SER | ILE | SER | LYS | MET | ILE | SER | ASP |
Entity 2, CPSF100 CTD1-CTD2 116 residues - Formula weight is not available
| 1 | MET | SER | ASP | VAL | SER | MET | GLY | MET | VAL | LYS | ||||
| 2 | LEU | ASP | LYS | GLY | PHE | ASP | ALA | LEU | ASN | TYR | ||||
| 3 | ARG | ALA | ILE | GLY | THR | ASP | SER | VAL | ALA | SER | ||||
| 4 | PHE | ARG | GLY | VAL | ARG | ASP | GLY | ASP | MET | VAL | ||||
| 5 | ARG | CYS | ILE | GLY | GLU | GLY | PRO | ARG | MET | VAL | ||||
| 6 | ILE | GLY | HIS | ALA | ASP | ILE | ASN | GLU | MET | ARG | ||||
| 7 | ARG | MET | ILE | VAL | GLU | GLY | SER | MET | ARG | VAL | ||||
| 8 | GLU | GLN | GLU | GLU | ASN | GLY | LEU | LEU | VAL | GLU | ||||
| 9 | ASP | CYS | VAL | TRP | ILE | ARG | VAL | SER | GLY | ASP | ||||
| 10 | GLY | VAL | THR | ILE | ASP | GLY | ARG | ASP | SER | GLY | ||||
| 11 | VAL | PHE | TYR | ALA | VAL | ARG | ASP | VAL | VAL | TYR | ||||
| 12 | ARG | SER | SER | ALA | PHE | ILE |
Samples:
sample_1: CPSF73, [U-99% 13C; U-99% 15N], 230 uM; CPSF100, [U-99% 13C; U-99% 15N], 230 uM; TRIS 20 mM; sodium chloride 150 mM; DTT 2 mM
sample_2: CPSF73, [U-15N; 99% 13C-Ile], 625 uM; CPSF100, [U-15N; 99% 13C-Ile], 625 uM; TRIS 20 mM; sodium chloride 150 mM; DTT 2 mM
sample_3: CPSF73, [U-15N; 99% 13C-Val], 150 uM; CPSF100, [U-15N; 99% 13C-Val], 150 uM; TRIS 20 mM; sodium chloride 150 mM; DTT 2 mM
sample_4: CPSF73, [U-15N; 99% 13C-Leu], 280 uM; CPSF100, [U-15N; 99% 13C-Leu], 280 uM; TRIS 20 mM; sodium chloride 150 mM; DTT 2 mM
sample_5: CPSF73, [U-10% 13C; U-100% 15N], 880 uM; CPSF100, [U-10% 13C; U-100% 15N], 880 uM; TRIS 20 mM; sodium chloride 150 mM; DTT 2 mM
sample_6: CPSF73 458 uM; CPSF100 458 uM; TRIS 20 mM; sodium chloride 150 mM; DTT 2 mM
sample_7: CPSF73, [95% 2H]-Phe, 150 uM; CPSF100, [95% 2H]-Phe, 150 uM; TRIS 20 mM; sodium chloride 150 mM; DTT 2 mM
sample_8: CPSF73, [95% 2H]-Tyr,Trp, 85 uM; CPSF100, [95% 2H]-Tyr,Trp, 85 uM; TRIS 20 mM; sodium chloride 150 mM; DTT 2 mM
sample_conditions_1: ionic strength: 170 mM; pH: 7.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N TROSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
| 3D HA(CACO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D N(COCA)NNH | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D TROSY-HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D TROSY-HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D (H)CCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D H(C)CH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D (H)C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C ct-HSQC methyl | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-13C ct-HSQC methyl | sample_3 | isotropic | sample_conditions_1 |
| 2D 1H-13C ct-HSQC methyl | sample_4 | isotropic | sample_conditions_1 |
| 3D TROSY-HNCO | sample_2 | isotropic | sample_conditions_1 |
| 3D TROSY-HNCO | sample_3 | isotropic | sample_conditions_1 |
| 3D TROSY-HNCO | sample_4 | isotropic | sample_conditions_1 |
| 3D TROSY-HNCA | sample_2 | isotropic | sample_conditions_1 |
| 3D TROSY-HNCA | sample_3 | isotropic | sample_conditions_1 |
| 3D TROSY-HNCA | sample_4 | isotropic | sample_conditions_1 |
| 3D (H)CCH-TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 3D (H)CCH-TOCSY | sample_3 | isotropic | sample_conditions_1 |
| 3D (H)CCH-TOCSY | sample_4 | isotropic | sample_conditions_1 |
| 2D 1H-13C ct-HSQC methyl | sample_5 | isotropic | sample_conditions_1 |
| 3D 1H-15N HSQC-TOCSY | sample_5 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_6 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_6 | isotropic | sample_conditions_1 |
| 2D 1H-1H DQF-COSY | sample_6 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_7 | isotropic | sample_conditions_1 |
| 2D 1H-1H DQF-COSY | sample_7 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_8 | isotropic | sample_conditions_1 |
| 2D 1H-1H DQF-COSY | sample_8 | isotropic | sample_conditions_1 |
| 3D H(C)CH-TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 3D H(C)CH-TOCSY | sample_3 | isotropic | sample_conditions_1 |
| 2D HN(CA) | sample_2 | isotropic | sample_conditions_1 |
| 2D HN(CA) | sample_3 | isotropic | sample_conditions_1 |
| 2D HN(CO) | sample_2 | isotropic | sample_conditions_1 |
| 2D HN(CO) | sample_3 | isotropic | sample_conditions_1 |
| 3D HSQC-NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v4.0 - collection
NMRPipe v8.6 - processing
SPARKY vNMRFAM - data analysis
SPARKY vNMRFAM - peak picking
SPARKY vNMRFAM - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 700 MHz
- Bruker AVANCE NEO 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts