BMRB Entry 51647
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51647
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Title: Backbone 1H, 13C and 15N chemical shift assignments of ubiquitin with a modified N-terminal twin-strep tag. PubMed: 37010382
Deposition date: 2022-10-03 Original release date: 2023-03-23
Authors: Nelson, Spencer; Deshmukh, Lalit
Citation: Nelson, Spencer; Li, Yunan; Chen, Yue; Deshmukh, Lalit. "Avidity-Based Method for the Efficient Generation of Monoubiquitinated Recombinant Proteins" J. Am. Chem. Soc. 145, 7748-7752 (2023).
Assembly members:
entity_1, polymer, 119 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11a
Entity Sequences (FASTA):
entity_1: GSWSHPQFERGGGSGGGSGG
SSAWSHPQFERGSENLYFQG
SGSMQIFVKTLTGKTITLEV
EPSDTIENVKAKIQDKEGIP
PDQQRLIFAGKQLEDGRTLS
DYNIQKESTLHLVLRLRGG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 180 |
| 15N chemical shifts | 91 |
| 1H chemical shifts | 91 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ubiquitin with modified N-terminal twin-strep tag | 1 |
Entities:
Entity 1, Ubiquitin with modified N-terminal twin-strep tag 119 residues - Formula weight is not available
| 1 | GLY | SER | TRP | SER | HIS | PRO | GLN | PHE | GLU | ARG | ||||
| 2 | GLY | GLY | GLY | SER | GLY | GLY | GLY | SER | GLY | GLY | ||||
| 3 | SER | SER | ALA | TRP | SER | HIS | PRO | GLN | PHE | GLU | ||||
| 4 | ARG | GLY | SER | GLU | ASN | LEU | TYR | PHE | GLN | GLY | ||||
| 5 | SER | GLY | SER | MET | GLN | ILE | PHE | VAL | LYS | THR | ||||
| 6 | LEU | THR | GLY | LYS | THR | ILE | THR | LEU | GLU | VAL | ||||
| 7 | GLU | PRO | SER | ASP | THR | ILE | GLU | ASN | VAL | LYS | ||||
| 8 | ALA | LYS | ILE | GLN | ASP | LYS | GLU | GLY | ILE | PRO | ||||
| 9 | PRO | ASP | GLN | GLN | ARG | LEU | ILE | PHE | ALA | GLY | ||||
| 10 | LYS | GLN | LEU | GLU | ASP | GLY | ARG | THR | LEU | SER | ||||
| 11 | ASP | TYR | ASN | ILE | GLN | LYS | GLU | SER | THR | LEU | ||||
| 12 | HIS | LEU | VAL | LEU | ARG | LEU | ARG | GLY | GLY |
Samples:
sample_1: Ubiquitin with modified N-terminal twin-strep tag, [U-100% 13C; U-100% 15N], 1 mM; D2O 7%; EDTA 1 mM; sodium phosphate 20 mM
sample_conditions_1: ionic strength: 0 M; pH: 6.5; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N TROSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N TROSY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe - data analysis
CcpNMR - data analysis
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker AVANCE NEO 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts