BMRB Entry 51651
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51651
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Title: Rev7/Rev3-RBM1 PubMed: 37129702
Deposition date: 2022-10-04 Original release date: 2023-06-23
Authors: Arianna, Gianluca; Geddes-Buehre, Dane; Korzhnev, Dmitry
Citation: Arianna, Gianluca; Geddes-Buehre, Dane; Korzhnev, Dmitry. "Backbone and ILV side-chain methyl NMR resonance assignments of human Rev7/Rev3-RBM1 and Rev7/Rev3-RBM2 complexes" Biomol. NMR Assign. 17, 107-114 (2023).
Assembly members:
entity_1, polymer, 211 residues, Formula weight is not available
entity_2, polymer, 27 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet-Duet-1
Entity Sequences (FASTA):
entity_1: MTTLTRQDLNFGQVVADVLC
EFLEVAVHLILYVREVYPVG
IFQKRKKYNVPVQMSCHPEL
NQYIQDTLHCVKPLLEKNDV
EKVVVVILDKEHRPVEKFVF
EITQPPLLSISSDSLLSHVE
QLLAAFILKISVCDAVLDHN
PPGCTFTVLVHTREAATRNM
EKIQVIKDFPWILADEQDVH
MHDPRLIPLKTMTSDILKMQ
LYVEERAHKGS
entity_2: MRTANILKPLMSPPSREEIM
ATLLDHD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 772 |
| 15N chemical shifts | 208 |
| 1H chemical shifts | 484 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Rev7 | 1 |
| 2 | Rev3-RBM1 | 2 |
Entities:
Entity 1, Rev7 211 residues - Formula weight is not available
| 1 | MET | THR | THR | LEU | THR | ARG | GLN | ASP | LEU | ASN | ||||
| 2 | PHE | GLY | GLN | VAL | VAL | ALA | ASP | VAL | LEU | CYS | ||||
| 3 | GLU | PHE | LEU | GLU | VAL | ALA | VAL | HIS | LEU | ILE | ||||
| 4 | LEU | TYR | VAL | ARG | GLU | VAL | TYR | PRO | VAL | GLY | ||||
| 5 | ILE | PHE | GLN | LYS | ARG | LYS | LYS | TYR | ASN | VAL | ||||
| 6 | PRO | VAL | GLN | MET | SER | CYS | HIS | PRO | GLU | LEU | ||||
| 7 | ASN | GLN | TYR | ILE | GLN | ASP | THR | LEU | HIS | CYS | ||||
| 8 | VAL | LYS | PRO | LEU | LEU | GLU | LYS | ASN | ASP | VAL | ||||
| 9 | GLU | LYS | VAL | VAL | VAL | VAL | ILE | LEU | ASP | LYS | ||||
| 10 | GLU | HIS | ARG | PRO | VAL | GLU | LYS | PHE | VAL | PHE | ||||
| 11 | GLU | ILE | THR | GLN | PRO | PRO | LEU | LEU | SER | ILE | ||||
| 12 | SER | SER | ASP | SER | LEU | LEU | SER | HIS | VAL | GLU | ||||
| 13 | GLN | LEU | LEU | ALA | ALA | PHE | ILE | LEU | LYS | ILE | ||||
| 14 | SER | VAL | CYS | ASP | ALA | VAL | LEU | ASP | HIS | ASN | ||||
| 15 | PRO | PRO | GLY | CYS | THR | PHE | THR | VAL | LEU | VAL | ||||
| 16 | HIS | THR | ARG | GLU | ALA | ALA | THR | ARG | ASN | MET | ||||
| 17 | GLU | LYS | ILE | GLN | VAL | ILE | LYS | ASP | PHE | PRO | ||||
| 18 | TRP | ILE | LEU | ALA | ASP | GLU | GLN | ASP | VAL | HIS | ||||
| 19 | MET | HIS | ASP | PRO | ARG | LEU | ILE | PRO | LEU | LYS | ||||
| 20 | THR | MET | THR | SER | ASP | ILE | LEU | LYS | MET | GLN | ||||
| 21 | LEU | TYR | VAL | GLU | GLU | ARG | ALA | HIS | LYS | GLY | ||||
| 22 | SER |
Entity 2, Rev3-RBM1 27 residues - Formula weight is not available
| 1 | MET | ARG | THR | ALA | ASN | ILE | LEU | LYS | PRO | LEU | ||||
| 2 | MET | SER | PRO | PRO | SER | ARG | GLU | GLU | ILE | MET | ||||
| 3 | ALA | THR | LEU | LEU | ASP | HIS | ASP |
Samples:
sample_1: Rev7R124A, [U-15N; U-13C; U-2H; 99% 13CH3,12CD3 D1/D2 Leu; 13CH3,12CD3 G1/G2 Val; 99% 13CH3 D1-Ile], 0.5 mM; Rev3-RBM1, [U-15N; U-13C; U-2H; 99% 13CH3,12CD3 D1/D2 Leu; 13CH3,12CD3 G1/G2 Val; 99% 13CH3 D1-Ile], 0.5 mM
sample_2: Rev7K44A,R124A,A135D, [U-15N; U-12C; U-2H; 99% 13CH3,12CD3 D1/D2 Leu; 13CH3,12CD3 G1/G2 Val; 99% 13CH3 D1-Ile], 0.75 mM; Rev3-RBM1, [U-15N; U-12C; U-2H; 99% 13CH3,12CD3 D1/D2 Leu; 13CH3,12CD3 G1/G2 Val; 99% 13CH3 D1-Ile], 0.75 mM
sample_conditions_1: ionic strength: 0.2 M; pH: 7.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N TROSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HMQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HMCMCGCBCA | sample_1 | isotropic | sample_conditions_1 |
| 4D 13C-1H HMQC-NOESY-TROSY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-15N TROSY | sample_2 | isotropic | sample_conditions_1 |
| 13C-13C HMQC-NOESY-HMQC | sample_2 | isotropic | sample_conditions_1 |
Software:
NMRFAM-SPARKY - chemical shift assignment
SPARTA+ - chemical shift calculation
NMRPipe - processing
TALOS-N - structure solution
NMR spectrometers:
- Varian INOVA 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts