BMRB Entry 51674
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51674
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Title: DinB-PAD PubMed: 37260088
Deposition date: 2022-10-28 Original release date: 2023-06-23
Authors: Okeke, Damasus; Burmann, Bjorn
Citation: Okeke, Damasus; Lidman, Jens; Matecko-Burmann, Irena; Burmann, Bjorn. "Thumb-domain dynamics modulate the functional repertoire of DNA-Polymerase IV (DinB)" Nucleic Acids Res. 51, 7036-7052 (2023).
Assembly members:
entity_1, polymer, 124 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28b-SUMO
Entity Sequences (FASTA):
entity_1: GIDERDVNSERLRKSVGVER
TMAEDIHHWSECEAIIERLY
PELERRLAKVKPDLLIARQG
VKLKFDDFQQTTQEHVWPRL
NKADLIATARKTWDERRGGR
GVRLVGLHVTLLDPQMERQL
VLGL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 363 |
| 15N chemical shifts | 118 |
| 1H chemical shifts | 627 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DinB-PAd | 1 |
Entities:
Entity 1, DinB-PAd 124 residues - Formula weight is not available
| 1 | GLY | ILE | ASP | GLU | ARG | ASP | VAL | ASN | SER | GLU | ||||
| 2 | ARG | LEU | ARG | LYS | SER | VAL | GLY | VAL | GLU | ARG | ||||
| 3 | THR | MET | ALA | GLU | ASP | ILE | HIS | HIS | TRP | SER | ||||
| 4 | GLU | CYS | GLU | ALA | ILE | ILE | GLU | ARG | LEU | TYR | ||||
| 5 | PRO | GLU | LEU | GLU | ARG | ARG | LEU | ALA | LYS | VAL | ||||
| 6 | LYS | PRO | ASP | LEU | LEU | ILE | ALA | ARG | GLN | GLY | ||||
| 7 | VAL | LYS | LEU | LYS | PHE | ASP | ASP | PHE | GLN | GLN | ||||
| 8 | THR | THR | GLN | GLU | HIS | VAL | TRP | PRO | ARG | LEU | ||||
| 9 | ASN | LYS | ALA | ASP | LEU | ILE | ALA | THR | ALA | ARG | ||||
| 10 | LYS | THR | TRP | ASP | GLU | ARG | ARG | GLY | GLY | ARG | ||||
| 11 | GLY | VAL | ARG | LEU | VAL | GLY | LEU | HIS | VAL | THR | ||||
| 12 | LEU | LEU | ASP | PRO | GLN | MET | GLU | ARG | GLN | LEU | ||||
| 13 | VAL | LEU | GLY | LEU |
Samples:
sample_1: DinB-PAD, [U-100% 13C; U-100% 15N], 500 uM; KPi 50 mM; KCl 100 mM; Arginine 50 mM; Glutamine 50 mM; EDTA 1 mM; TCEP 1 mM; CHAPSO 0.1 mM; D2O 10%
sample_conditions_1: ionic strength: 200 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D (H)CCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v3.6 - collection
NMRPipe - processing
CARA - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 700 MHz
- Bruker AVANCE III 800 MHz
- Bruker AVANCE III 900 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts