BMRB Entry 51695
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51695
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Chemical shift assignment of the intracellular domain of the prolactin receptor, residues 236-396 PubMed: 37232489
Deposition date: 2022-11-14 Original release date: 2023-06-06
Authors: Haxholm, Gitte; Seiffert, Pernille; Kragelund, Birthe
Citation: Araya-Secchi, Raul; Bugge, Katrine; Seiffert, Pernille; Petry, Amalie; Haxholm, Gitte; Lindorff-Larsen, Kresten; Pedersen, Stine; Arleth, Lise; Kragelund, Birthe. "The prolactin receptor scaffolds Janus kinase 2 via co-structure formation with phosphoinositide-4,5-bisphosphate" eLife 12, e84645-e84645 (2023).
Assembly members:
entity_1, polymer, 161 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11a
Entity Sequences (FASTA):
entity_1: GYSMVTCIFPPVPGPKIKGF
DAHLLEKGKSEELLSALGCQ
DFPPTSDYEDLLVEYLEVDD
SEDQHLMSVHSKEHPSQGMK
PTYLDPDTDSGRGSCDSPSL
LSEKCEEPQANPSTFYDPEV
IEKPENPETTHTWDPQCISM
EGKIPYFHAGGSKCSTWPLP
Q
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 453 |
15N chemical shifts | 136 |
1H chemical shifts | 136 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | PRLR-ICD(236-396) | 1 |
Entities:
Entity 1, PRLR-ICD(236-396) 161 residues - Formula weight is not available
1 | GLY | TYR | SER | MET | VAL | THR | CYS | ILE | PHE | PRO | ||||
2 | PRO | VAL | PRO | GLY | PRO | LYS | ILE | LYS | GLY | PHE | ||||
3 | ASP | ALA | HIS | LEU | LEU | GLU | LYS | GLY | LYS | SER | ||||
4 | GLU | GLU | LEU | LEU | SER | ALA | LEU | GLY | CYS | GLN | ||||
5 | ASP | PHE | PRO | PRO | THR | SER | ASP | TYR | GLU | ASP | ||||
6 | LEU | LEU | VAL | GLU | TYR | LEU | GLU | VAL | ASP | ASP | ||||
7 | SER | GLU | ASP | GLN | HIS | LEU | MET | SER | VAL | HIS | ||||
8 | SER | LYS | GLU | HIS | PRO | SER | GLN | GLY | MET | LYS | ||||
9 | PRO | THR | TYR | LEU | ASP | PRO | ASP | THR | ASP | SER | ||||
10 | GLY | ARG | GLY | SER | CYS | ASP | SER | PRO | SER | LEU | ||||
11 | LEU | SER | GLU | LYS | CYS | GLU | GLU | PRO | GLN | ALA | ||||
12 | ASN | PRO | SER | THR | PHE | TYR | ASP | PRO | GLU | VAL | ||||
13 | ILE | GLU | LYS | PRO | GLU | ASN | PRO | GLU | THR | THR | ||||
14 | HIS | THR | TRP | ASP | PRO | GLN | CYS | ILE | SER | MET | ||||
15 | GLU | GLY | LYS | ILE | PRO | TYR | PHE | HIS | ALA | GLY | ||||
16 | GLY | SER | LYS | CYS | SER | THR | TRP | PRO | LEU | PRO | ||||
17 | GLN |
Samples:
sample_1: sodium phosphate 20 mM; PRLR-ICD(236-396), [U-100% 13C; U-100% 15N], 650 uM; D2O 10%; TCEP 6.5 mM; DSS 0.5 mM
sample_conditions_1: pH: 7.3; pressure: 1 atm; temperature: 277.15 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR - data analysis
NMR spectrometers:
- Varian INOVA 750 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts