BMRB Entry 51696

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51696

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Title:
Backbone chemical shift assignment of yeast expressed NIST-Fab light and heavy chain
Deposition date:
2022-11-15
Original release date:
2023-03-01
Authors:
Solomon, Tsega; Brinson, Robert
Citation:

Citation: Solomon, Tsega; Chao, Kinlin; Gingras, Genevieve; Aubin, Yves; O'Dell, W. Brad; Marino, John; Brinson, Robert. "Backbone NMR assignment of the yeast expressed Fab fragment of the NISTmAb reference antibody"  Biomol. NMR Assign. 17, 75-81 (2023).
PubMed: 36856943

Assembly members:

Assembly members:
entity_1, polymer, 217 residues, Formula weight is not available
entity_2, polymer, 231 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Pichia pastoris   Vector: pPICZ-A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: EAEADIQMTQSPSTLSASVG DRVTITCSASSRVGYMHWYQ QKPGKAPKLLIYDTSKLASG VPSRFSGSGSGTEFTLTISS LQPDDFATYYCFQGSGYPFT FGGGTKVEIKRTVAAPSVFI FPPSDEQLKSGTASVVCLLN NFYPREAKVQWKVDNALQSG NSQESVTEQDSKDSTYSLSS TLTLSKADYEKHKVYACEVT HQGLSSPVTKSFNRGEC
entity_2: EAEAQVTLRESGPALVKPTQ TLTLTCTFSGFSLSTAGMSV GWIRQPPGKALEWLADIWWD DKKHYNPSLKDRLTISKDTS KNQVVLKVTNMDPADTATYY CARDMIFNFYFDVWGQGTTV TVSSASTKGPSVFPLAPSSK STSGGTAALGCLVKDYFPEP VTVSWNSGALTSGVHTFPAV LQSSGLYSLSSVVTVPSSSL GTQTYICNVNHKPSNTKVDK RVEPKSCDKTH

Data sets:
Data typeCount
13C chemical shifts1231
15N chemical shifts398
1H chemical shifts398

Additional metadata:

  • Assembly
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Assembly:

Entity Assembly IDEntity NameEntity ID
1NIST-Fab, Chain 11
2NIST-Fab, Chain 22

Entities:

Entity 1, NIST-Fab, Chain 1 217 residues - Formula weight is not available

Residue 1 begins at D

1   GLUALAGLUALAASPILEGLNMETTHRGLN
2   SERPROSERTHRLEUSERALASERVALGLY
3   ASPARGVALTHRILETHRCYSSERALASER
4   SERARGVALGLYTYRMETHISTRPTYRGLN
5   GLNLYSPROGLYLYSALAPROLYSLEULEU
6   ILETYRASPTHRSERLYSLEUALASERGLY
7   VALPROSERARGPHESERGLYSERGLYSER
8   GLYTHRGLUPHETHRLEUTHRILESERSER
9   LEUGLNPROASPASPPHEALATHRTYRTYR
10   CYSPHEGLNGLYSERGLYTYRPROPHETHR
11   PHEGLYGLYGLYTHRLYSVALGLUILELYS
12   ARGTHRVALALAALAPROSERVALPHEILE
13   PHEPROPROSERASPGLUGLNLEULYSSER
14   GLYTHRALASERVALVALCYSLEULEUASN
15   ASNPHETYRPROARGGLUALALYSVALGLN
16   TRPLYSVALASPASNALALEUGLNSERGLY
17   ASNSERGLNGLUSERVALTHRGLUGLNASP
18   SERLYSASPSERTHRTYRSERLEUSERSER
19   THRLEUTHRLEUSERLYSALAASPTYRGLU
20   LYSHISLYSVALTYRALACYSGLUVALTHR
21   HISGLNGLYLEUSERSERPROVALTHRLYS
22   SERPHEASNARGGLYGLUCYS

Entity 2, NIST-Fab, Chain 2 231 residues - Formula weight is not available

Residue 1 begins at Q

1   GLUALAGLUALAGLNVALTHRLEUARGGLU
2   SERGLYPROALALEUVALLYSPROTHRGLN
3   THRLEUTHRLEUTHRCYSTHRPHESERGLY
4   PHESERLEUSERTHRALAGLYMETSERVAL
5   GLYTRPILEARGGLNPROPROGLYLYSALA
6   LEUGLUTRPLEUALAASPILETRPTRPASP
7   ASPLYSLYSHISTYRASNPROSERLEULYS
8   ASPARGLEUTHRILESERLYSASPTHRSER
9   LYSASNGLNVALVALLEULYSVALTHRASN
10   METASPPROALAASPTHRALATHRTYRTYR
11   CYSALAARGASPMETILEPHEASNPHETYR
12   PHEASPVALTRPGLYGLNGLYTHRTHRVAL
13   THRVALSERSERALASERTHRLYSGLYPRO
14   SERVALPHEPROLEUALAPROSERSERLYS
15   SERTHRSERGLYGLYTHRALAALALEUGLY
16   CYSLEUVALLYSASPTYRPHEPROGLUPRO
17   VALTHRVALSERTRPASNSERGLYALALEU
18   THRSERGLYVALHISTHRPHEPROALAVAL
19   LEUGLNSERSERGLYLEUTYRSERLEUSER
20   SERVALVALTHRVALPROSERSERSERLEU
21   GLYTHRGLNTHRTYRILECYSASNVALASN
22   HISLYSPROSERASNTHRLYSVALASPLYS
23   ARGVALGLUPROLYSSERCYSASPLYSTHR
24   HIS

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks