BMRB Entry 51709
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51709
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Title: SARS-CoV-2 Nucleocapsid 251-419 PubMed: 36807437
Deposition date: 2022-11-26 Original release date: 2023-03-07
Authors: Lee, Eunjeong
Citation: Lee, Eunjeong; Redzic, Jasmina; Saviola, Anthony; Li, Xueni; Ebmeier, Christopher; Kutateladze, Tatiana; Hansen, Kirk Charles; Zhao, Rui; Ahn, Natalie; Sluchanko, Nikolai; Eisenmesser, Elan. "Molecular insight into the specific interactions of the SARS-CoV-2 Nucleocapsid with RNA and host protein" Protein Sci 32, e4603-e4603 (2023).
Assembly members:
entity_1, polymer, 169 residues, Formula weight is not available
Natural source: Common Name: SARS-CoV-2 Taxonomy ID: 2697049 Superkingdom: Viruses Kingdom: not available Genus/species: Betacoronavirus HCoV-SARS
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET29
Entity Sequences (FASTA):
entity_1: AAEASKKPRQKRTATKAYNV
TQAFGRRGPEQTQGNFGDQE
LIRQGTDYKHWPQIAQFAPS
ASAFFGMSRIGMEVTPSGTW
LTYTGAIKLDDKDPNFKDQV
ILLNKHIDAYKTFPPTEPKK
DKKKKADETQALPQRQKKQQ
TVTLLPAADLDDFSKQLQQS
MSSADSTQA
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 271 |
15N chemical shifts | 128 |
1H chemical shifts | 128 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Nucleocapsid 251-419 | 1 |
Entities:
Entity 1, Nucleocapsid 251-419 169 residues - Formula weight is not available
1 | ALA | ALA | GLU | ALA | SER | LYS | LYS | PRO | ARG | GLN | ||||
2 | LYS | ARG | THR | ALA | THR | LYS | ALA | TYR | ASN | VAL | ||||
3 | THR | GLN | ALA | PHE | GLY | ARG | ARG | GLY | PRO | GLU | ||||
4 | GLN | THR | GLN | GLY | ASN | PHE | GLY | ASP | GLN | GLU | ||||
5 | LEU | ILE | ARG | GLN | GLY | THR | ASP | TYR | LYS | HIS | ||||
6 | TRP | PRO | GLN | ILE | ALA | GLN | PHE | ALA | PRO | SER | ||||
7 | ALA | SER | ALA | PHE | PHE | GLY | MET | SER | ARG | ILE | ||||
8 | GLY | MET | GLU | VAL | THR | PRO | SER | GLY | THR | TRP | ||||
9 | LEU | THR | TYR | THR | GLY | ALA | ILE | LYS | LEU | ASP | ||||
10 | ASP | LYS | ASP | PRO | ASN | PHE | LYS | ASP | GLN | VAL | ||||
11 | ILE | LEU | LEU | ASN | LYS | HIS | ILE | ASP | ALA | TYR | ||||
12 | LYS | THR | PHE | PRO | PRO | THR | GLU | PRO | LYS | LYS | ||||
13 | ASP | LYS | LYS | LYS | LYS | ALA | ASP | GLU | THR | GLN | ||||
14 | ALA | LEU | PRO | GLN | ARG | GLN | LYS | LYS | GLN | GLN | ||||
15 | THR | VAL | THR | LEU | LEU | PRO | ALA | ALA | ASP | LEU | ||||
16 | ASP | ASP | PHE | SER | LYS | GLN | LEU | GLN | GLN | SER | ||||
17 | MET | SER | SER | ALA | ASP | SER | THR | GLN | ALA |
Samples:
sample_1: Nucleocapsid 251-419, [U-13C; U-15N; U-2H], 500 uM; sodium chloride 150 mM; HEPES 50 mM
sample_conditions_1: ionic strength: 150 mM; pH: 7; pressure: 1 atm; temperature: 308 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR - data analysis
NMR spectrometers:
- Bruker AVANCE NEO 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts