BMRB Entry 51731
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51731
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Title: Cerato Ulmin PubMed: 36768573
Deposition date: 2022-12-13 Original release date: 2023-03-07
Authors: Gallo, Mariana; Spisni, Alberto; Pertinhez, Thelma
Citation: Gallo, Mariana; Luti, Simone; Baroni, Fabio; Baccelli, Ivan; Cilli, Eduardo Maffud; Cicchi, Costanza; Leri, Manuela; Spisni, Alberto; Pertinhez, Thelma; Pazzagli, Luigia. "Plant Defense Elicitation by the Hydrophobin Cerato-Ulmin and Correlation with Its Structural Features" Int. J. Mol. Sci. 24, 2251-2251 (2023).
Assembly members:
entity_1, polymer, 79 residues, Formula weight is not available
Natural source: Common Name: Ophiostoma ulmi Taxonomy ID: 5174 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Ophiostoma ulmi
Experimental source: Production method: recombinant technology Host organism: Pichia pastoris Vector: E. coli DH5alpha
Entity Sequences (FASTA):
entity_1: EAEASDSYDPCTGLLQKSPQ
CCNTDILGVANLDCHGPPSV
PTSPSQFQASCVADGGRSAR
CCTLSLLGLALVCTDPVGI
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 64 |
| 1H chemical shifts | 323 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | cerato-ulmin | 1 |
Entities:
Entity 1, cerato-ulmin 79 residues - Formula weight is not available
| 1 | GLU | ALA | GLU | ALA | SER | ASP | SER | TYR | ASP | PRO | ||||
| 2 | CYS | THR | GLY | LEU | LEU | GLN | LYS | SER | PRO | GLN | ||||
| 3 | CYS | CYS | ASN | THR | ASP | ILE | LEU | GLY | VAL | ALA | ||||
| 4 | ASN | LEU | ASP | CYS | HIS | GLY | PRO | PRO | SER | VAL | ||||
| 5 | PRO | THR | SER | PRO | SER | GLN | PHE | GLN | ALA | SER | ||||
| 6 | CYS | VAL | ALA | ASP | GLY | GLY | ARG | SER | ALA | ARG | ||||
| 7 | CYS | CYS | THR | LEU | SER | LEU | LEU | GLY | LEU | ALA | ||||
| 8 | LEU | VAL | CYS | THR | ASP | PRO | VAL | GLY | ILE |
Samples:
sample_1: Cerato-ulmin, [U-100% 15N], 700 ± 20 uM; sodium phosphate 35 ± 3 mM; acetonitrile, [U-2H], 30 ± 5 %
sample_conditions_1: ionic strength: 38 mM; pH: 5.8; pressure: 1 atm; temperature: 288.0 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1D 1H | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRViewJ v9.2 - chemical shift assignment, data analysis, peak picking
NMRPipe - processing
NMR spectrometers:
- Varian INOVA 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts