BMRB Entry 51772

Name: CTADN region of NAP1
Published: 2023-06-30

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51772

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: CTADN region of NAP1 PubMed: 37380014

Deposition date: 2023-01-09 Original release date: 2023-06-30

Authors: Ohtomo, Hideaki; Kurita, Junichi; Nishimura, Yoshifumi

Citation: Ohtomo, Hideaki; Yamane, Tsutomu; Oda, Takashi; Kodera, Noriyuki; Kurita, Junichi; Tsunaka, Yasuo; Amyot, Romain; Ikeguchi, Mutsunori; Nishimura, Yoshifumi. "Dynamic solution structures of whole human NAP1 dimer bound to one and two histone H2A-H2B heterodimers obtained by integrative methods" J. Mol. Biol. 435, 168189-168189 (2023).

Assembly members:
entity_1, polymer, 25 residues, Formula weight is not available

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET

Entity Sequences (FASTA):
entity_1: LGSGEAIEDDDDDYDEEGEE ADEEG

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	2
15N chemical shifts	22
1H chemical shifts	24

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CTADN	1

Entities:

Entity 1, CTADN 25 residues - Formula weight is not available

1

LEU

GLY

SER

GLY

GLU

ALA

ILE

GLU

ASP

2

ASP

TYR

ASP

GLU

GLY

GLU

3

ALA

ASP

GLU

GLY

Samples:

sample_1: CTADN of NAP1, [U-99% 15N], 475 uM

sample_conditions_1: ionic strength: 0.2 M; pH: 6.0; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1

Software:

Olivia - chemical shift assignment

NMR spectrometers:

Bruker AVANCE III 950 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts