BMRB Entry 51782

Name: His6-AviTag-Sox2_aa234-317_C265A
Published: 2023-01-18

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51782

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: His6-AviTag-Sox2_aa234-317_C265A

Deposition date: 2023-01-10 Original release date: 2023-01-18

Authors: Theillet, Francois-Xavier

Citation: Theillet, Francois-Xavier. "Structural characterization of Oct4, Sox2, Nanog and Esrrb disordered domains, and a method to identify their phospho-dependent binding partners." C. R. Chim. ., .-..

Assembly members:
entity_1, polymer, 112 residues, Formula weight is not available

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet45b(+)

Entity Sequences (FASTA):
entity_1: MAHHHHHHVGTGLNDIFEAQ KIEWHEGAGMALGSMGSVVK SEASSSPPVVTSSSHSRAPA QAGDLRDMISMYLPGAEVPE PAAPSRLHMSQHYQSGPVPG TAINGTLPLSHM

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	294
15N chemical shifts	92
1H chemical shifts	92

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	His6-AviTag-Sox2 aa234-317 C265A	1

Entities:

Entity 1, His6-AviTag-Sox2 aa234-317 C265A 112 residues - Formula weight is not available

MAHHHHHHVGTGLNDIFEAQKIEWHEGA corresponds to His6-AviTag-, GMALGSMGSVVKSEASSSPPVVTSSSHSRAPAQAGDLRDMISMYLPGAEVPEPAAPSRLHMSQHYQSGPVPGTAINGTLPLSHM corresponds to human Sox2_aa234-317_C265A.

1

MET

ALA

HIS

VAL

GLY

2

THR

GLY

LEU

ASN

ASP

ILE

PHE

GLU

ALA

GLN

3

LYS

ILE

GLU

TRP

HIS

GLU

GLY

ALA

GLY

MET

4

ALA

LEU

GLY

SER

MET

GLY

SER

VAL

LYS

5

SER

GLU

ALA

SER

PRO

VAL

6

THR

SER

HIS

SER

ARG

ALA

PRO

ALA

7

GLN

ALA

GLY

ASP

LEU

ARG

ASP

MET

ILE

SER

8

MET

TYR

LEU

PRO

GLY

ALA

GLU

VAL

PRO

GLU

9

PRO

ALA

PRO

SER

ARG

LEU

HIS

MET

SER

10

GLN

HIS

TYR

GLN

SER

GLY

PRO

VAL

PRO

GLY

11

THR

ALA

ILE

ASN

GLY

THR

LEU

PRO

LEU

SER

12

HIS

MET

Samples:

sample_1: His6-AviTag-Sox2_aa234-317_C265A, [U-98% 13C; U-98% 15N], 600 ± 100 uM; DSS 0.1 mM; HEPES 20 mM; sodium chloride 50 mM; cocktail protease inhibitors 2 tablet/100mL

sample_conditions_1: ionic strength: 70 mM; pH: 6.8; pressure: 1 atm; temperature: 283 K

Experiments:

Name	Sample	Sample state	Sample conditions
1D 1H	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D H(NCA)NH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3 - collection, processing

CcpNMR v2.1 - chemical shift assignment

NMR spectrometers:

Bruker Avance II 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts