BMRB Entry 51810

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51810

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Backbone chemical shifts for the Drosophila melanogaster Deltex WWE2 domain
Deposition date:
2023-01-25
Original release date:
2023-06-19
Authors:
Carter, Andrea; Majumdar, Ananya; Barrick, Doug
Citation:

Citation: Carter, Andrea; Ramsey, Kristen; Hatem, Christine; Sherry, Kathryn; Majumdar, Ananya; Barrick, Doug. "Structural features of the Notch ankyrin domain-Deltex WWE2 domain heterodimer determined by NMR spectroscopy and functional implications"  Structure 31, 584-594 (2023).
PubMed: 36977409

Assembly members:

Assembly members:
entity_1, polymer, 236 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: ATMALSTAGSGGPPVNHAHA VSVWEFESRGKWLPYSPAVS QHLERAHAKKLTRVMLSDAD PSLEQYYVNVRTMTQESEAE TAGSGLLTIGVRRMFYAPSS PAGKGTKWEWSGGSADSNND WRPYNMHVQSIIEDAWARGE QTLDLSNTHIGLPYTINFSN LTQLRQPSGPMRSIRRTQQA PYPLVKLTPQQANQLKSNSA SVSSQYNTLPKLGDTKSLHR VPMTRQQHPLPTSHQV

Data sets:
Data typeCount
13C chemical shifts479
15N chemical shifts169
1H chemical shifts169

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Deltex1

Entities:

Entity 1, Deltex 236 residues - Formula weight is not available

The first residue (A) is residue 26 of Drosophila Deltex. The last residue (V) is residue 261. Four surface-exposed Cysteine residues in Drosophila Deltex were substituted with Serine residues.

1   ALATHRMETALALEUSERTHRALAGLYSER
2   GLYGLYPROPROVALASNHISALAHISALA
3   VALSERVALTRPGLUPHEGLUSERARGGLY
4   LYSTRPLEUPROTYRSERPROALAVALSER
5   GLNHISLEUGLUARGALAHISALALYSLYS
6   LEUTHRARGVALMETLEUSERASPALAASP
7   PROSERLEUGLUGLNTYRTYRVALASNVAL
8   ARGTHRMETTHRGLNGLUSERGLUALAGLU
9   THRALAGLYSERGLYLEULEUTHRILEGLY
10   VALARGARGMETPHETYRALAPROSERSER
11   PROALAGLYLYSGLYTHRLYSTRPGLUTRP
12   SERGLYGLYSERALAASPSERASNASNASP
13   TRPARGPROTYRASNMETHISVALGLNSER
14   ILEILEGLUASPALATRPALAARGGLYGLU
15   GLNTHRLEUASPLEUSERASNTHRHISILE
16   GLYLEUPROTYRTHRILEASNPHESERASN
17   LEUTHRGLNLEUARGGLNPROSERGLYPRO
18   METARGSERILEARGARGTHRGLNGLNALA
19   PROTYRPROLEUVALLYSLEUTHRPROGLN
20   GLNALAASNGLNLEULYSSERASNSERALA
21   SERVALSERSERGLNTYRASNTHRLEUPRO
22   LYSLEUGLYASPTHRLYSSERLEUHISARG
23   VALPROMETTHRARGGLNGLNHISPROLEU
24   PROTHRSERHISGLNVAL

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks