BMRB Entry 51832
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51832
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Title: NMR Resonance Assignment of a Fibroblast Growth Factor 8 splicing isoform b. PubMed: 37118562
Deposition date: 2023-02-16 Original release date: 2023-05-23
Authors: Hargittay, Bruno; Mineev, Konstantin; Richter, Christian; Jonker, Hendrik; Saxena, Krishna; Schwalbe, Harald
Citation: Hargittay, Bruno; Mineev, Konstantin; Richter, Christian; Sreeramulu, Sridhar; Jonker, Hendrik; Saxena, Krishna; Schwalbe, Harald. "NMR resonance assignment of a fibroblast growth factor 8 splicing isoform b" Biomol. NMR Assignments 17, 135-142 (2023).
Assembly members:
entity_1, polymer, 168 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pKMProtG
Entity Sequences (FASTA):
entity_1: GAMGQVTVQSSPNFTQHVRE
QSLVTDQLSRRLIRTYQLYS
RTSGKHVQVLANKRINAMAE
DGDPFAKLIVETDTFGSRVR
VRGAETGLYICMNKKGKLIA
KSNGKGKDCVFTEIVLENNY
TALQNAKYEGWYMAFTRKGR
PRKGSKTRQHQREVHFMKRL
PRGHHTTE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 660 |
| 15N chemical shifts | 170 |
| 1H chemical shifts | 1112 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | FGF8b | 1 |
Entities:
Entity 1, FGF8b 168 residues - Formula weight is not available
| 1 | GLY | ALA | MET | GLY | GLN | VAL | THR | VAL | GLN | SER | ||||
| 2 | SER | PRO | ASN | PHE | THR | GLN | HIS | VAL | ARG | GLU | ||||
| 3 | GLN | SER | LEU | VAL | THR | ASP | GLN | LEU | SER | ARG | ||||
| 4 | ARG | LEU | ILE | ARG | THR | TYR | GLN | LEU | TYR | SER | ||||
| 5 | ARG | THR | SER | GLY | LYS | HIS | VAL | GLN | VAL | LEU | ||||
| 6 | ALA | ASN | LYS | ARG | ILE | ASN | ALA | MET | ALA | GLU | ||||
| 7 | ASP | GLY | ASP | PRO | PHE | ALA | LYS | LEU | ILE | VAL | ||||
| 8 | GLU | THR | ASP | THR | PHE | GLY | SER | ARG | VAL | ARG | ||||
| 9 | VAL | ARG | GLY | ALA | GLU | THR | GLY | LEU | TYR | ILE | ||||
| 10 | CYS | MET | ASN | LYS | LYS | GLY | LYS | LEU | ILE | ALA | ||||
| 11 | LYS | SER | ASN | GLY | LYS | GLY | LYS | ASP | CYS | VAL | ||||
| 12 | PHE | THR | GLU | ILE | VAL | LEU | GLU | ASN | ASN | TYR | ||||
| 13 | THR | ALA | LEU | GLN | ASN | ALA | LYS | TYR | GLU | GLY | ||||
| 14 | TRP | TYR | MET | ALA | PHE | THR | ARG | LYS | GLY | ARG | ||||
| 15 | PRO | ARG | LYS | GLY | SER | LYS | THR | ARG | GLN | HIS | ||||
| 16 | GLN | ARG | GLU | VAL | HIS | PHE | MET | LYS | ARG | LEU | ||||
| 17 | PRO | ARG | GLY | HIS | HIS | THR | THR | GLU |
Samples:
sample_1: FGF8b, [U-100% 13C; U-100% 15N], 400 uM; NaPi 50 mM; NaCl 300 mM
sample_conditions_1: ionic strength: 350 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
| 3D H(CCO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY aliphatic | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v4.1.1 - collection
SPARKY - chemical shift assignment
POKY - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 900 MHz
- Bruker AVANCE III 950 MHz
- Bruker AVANCE III 700 MHz
- Bruker AVANCE III 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts