BMRB Entry 51940
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51940
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Title: Backbone Assignment of Human Polymerase Theta MOTIF 2 phosphorylated by PLK1 PubMed: 37674080
Deposition date: 2023-04-26 Original release date: 2023-10-03
Authors: Zinn-Justin, Sophie; Miron, Simona; Boeffard-Dosierre, Liza; Airs-Loeuilliette, Julie
Citation: Gelot, Camille; Kovacs, Marton Tibor; Miron, Simona; Mylne, Emilie; Haan, Alexis; Boeffard-Dosierre, Liza; Ghouil, Rania; Popova, Tatiana; Dingli, Florent; Loew, Damarys; Guirouilh-Barbat, Josee; Del Nery, Elaine; Zinn-Justin, Sophie; Ceccaldi, Raphael. "Polth is phosphorylated by PLK1 to repair double-strand breaks in mitosis" Nature 621, 415-422 (2023).
Assembly members:
entity_1, polymer, 121 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-22b
Entity Sequences (FASTA):
entity_1: ENQDTHQQLTQXNDEXIIFS
EMDXVQMVEALDNVDIFPVQ
EKNHZVVSPRALELXDPVLD
EHHQGDQDGGDQDERAEKSK
LTGTRQNHXFIWSGAXFDLS
PGLQRILDKVSSPLENEKLK
S
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 340 |
| 15N chemical shifts | 111 |
| 1H chemical shifts | 111 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Human Polymerase Theta MOTIF 2 phosphorylated by PLK1 | 1 |
Entities:
Entity 1, Human Polymerase Theta MOTIF 2 phosphorylated by PLK1 121 residues - Formula weight is not available
| 1 | GLU | ASN | GLN | ASP | THR | HIS | GLN | GLN | LEU | THR | ||||
| 2 | GLN | SEP | ASN | ASP | GLU | SEP | ILE | ILE | PHE | SER | ||||
| 3 | GLU | MET | ASP | SEP | VAL | GLN | MET | VAL | GLU | ALA | ||||
| 4 | LEU | ASP | ASN | VAL | ASP | ILE | PHE | PRO | VAL | GLN | ||||
| 5 | GLU | LYS | ASN | HIS | TPO | VAL | VAL | SER | PRO | ARG | ||||
| 6 | ALA | LEU | GLU | LEU | SEP | ASP | PRO | VAL | LEU | ASP | ||||
| 7 | GLU | HIS | HIS | GLN | GLY | ASP | GLN | ASP | GLY | GLY | ||||
| 8 | ASP | GLN | ASP | GLU | ARG | ALA | GLU | LYS | SER | LYS | ||||
| 9 | LEU | THR | GLY | THR | ARG | GLN | ASN | HIS | SEP | PHE | ||||
| 10 | ILE | TRP | SER | GLY | ALA | SEP | PHE | ASP | LEU | SER | ||||
| 11 | PRO | GLY | LEU | GLN | ARG | ILE | LEU | ASP | LYS | VAL | ||||
| 12 | SER | SER | PRO | LEU | GLU | ASN | GLU | LYS | LEU | LYS | ||||
| 13 | SER |
Samples:
sample_1: Human Polymerase Theta MOTIF 2 phosphorylated by PLK1, [U-95% 13C; U-95% 15N], 300 uM
sample_conditions_1: ionic strength: 0.05 M; pH: 7; pressure: 1 atm; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCANNH | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts