BMRB Entry 51951

Name: Chemical Shift Assignments for ZNF750-Z*
Published: 2023-08-16

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PDB ID: 8sxm
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51951
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Chemical Shift Assignments for ZNF750-Z PubMed: 37655311*

Deposition date: 2023-05-03 Original release date: 2023-08-16

Authors: Rua, Antonio; Alexandrescu, Andrei

Citation: Rua, Antonio; Whitehead, Richard; Alexandrescu, Andrei. "NMR structure verifies the eponymous zinc finger domain of transcription factor ZNF750" J. Struc. Biol. 8, 100093-100093 (2023).

Assembly members:
entity_1, polymer, 27 residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: YKCFQCPFTCNEKSHLFNHM KYGLCKN

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	91
15N chemical shifts	30
1H chemical shifts	186

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ZNF750	1
2	zinc ion	2

Entities:

Entity 1, ZNF750 27 residues - Formula weight is not available

1

TYR

LYS

CYS

PHE

GLN

CYS

PRO

PHE

THR

CYS

2

ASN

GLU

LYS

SER

HIS

LEU

PHE

ASN

HIS

MET

3

LYS

TYR

GLY

LEU

CYS

LYS

ASN

Entity 2, zinc ion - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: ZNF750 1.9 mM; zinc 2.9 mM; sodium phosphate 10 mM

sample_2: ZNF750 3.8 mM; ZnCl2 4.8 mM; sodium phosphate 20 mM

sample_conditions_1: ionic strength: 1.9 mM; pH: 5.9; pressure: 1 atm; temperature: 288 K

sample_conditions_2: ionic strength: 3.8 mM; pH: 5.9; pressure: 1 atm; temperature: 288 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_2
2D DQF-COSY	sample_2	isotropic	sample_conditions_2
2D 1H-1H COSY	sample_2	isotropic	sample_conditions_2
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_2
2D 1H-1H TOCSY	sample_2	isotropic	sample_conditions_2

Software:

CcpNMR v2.5.2 - chemical shift assignment, peak picking

TOPSPIN v2.1 - NMR data collection

Molmol - structure visualization

X-PLOR NIH - structure solution

NMR spectrometers:

Bruker Avance 600 MHz MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts