BMRB Entry 51975
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51975
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Title: Chemical shift assignments for the sixth and seventh (HhH)2 repeats from Topoisomerase V PubMed: 37262333
Deposition date: 2023-05-23 Original release date: 2023-08-19
Authors: Petersen, Mark; Fang, Rebecca; Majumdar, Ananya; Barrick, Doug
Citation: Petersen, Mark; Fang, Rebecca; Majumdar, Ananya; Barrick, Doug. "Stability Islands and the Folding Cooperativity of a Seven-Repeat Array from Topoisomerase V" J. Am. Chem. Soc. 145, 12641-12650 (2023).
Assembly members:
entity_1, polymer, 135 residues, Formula weight is not available
Natural source: Common Name: Methanopyrus kandleri Taxonomy ID: 2320 Superkingdom: Archaea Kingdom: not available Genus/species: Methanopyrus kandleri
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pMal
Entity Sequences (FASTA):
entity_1: GGSWGIPKKLREAFDLETAA
ELYERYGSLKEIGRRLSYDD
LLELGATPKAAAEIKGPEFK
FLLNIEGVGPKLAERILEAV
DYDLERLASLNPEELAEKVE
GLGEELAERVVYAARERVES
RRKSGRQERSEEEWK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 369 |
| 15N chemical shifts | 200 |
| 1H chemical shifts | 200 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Topoisomerase V repeats FG | 1 |
Entities:
Entity 1, Topoisomerase V repeats FG 135 residues - Formula weight is not available
| 1 | GLY | GLY | SER | TRP | GLY | ILE | PRO | LYS | LYS | LEU | ||||
| 2 | ARG | GLU | ALA | PHE | ASP | LEU | GLU | THR | ALA | ALA | ||||
| 3 | GLU | LEU | TYR | GLU | ARG | TYR | GLY | SER | LEU | LYS | ||||
| 4 | GLU | ILE | GLY | ARG | ARG | LEU | SER | TYR | ASP | ASP | ||||
| 5 | LEU | LEU | GLU | LEU | GLY | ALA | THR | PRO | LYS | ALA | ||||
| 6 | ALA | ALA | GLU | ILE | LYS | GLY | PRO | GLU | PHE | LYS | ||||
| 7 | PHE | LEU | LEU | ASN | ILE | GLU | GLY | VAL | GLY | PRO | ||||
| 8 | LYS | LEU | ALA | GLU | ARG | ILE | LEU | GLU | ALA | VAL | ||||
| 9 | ASP | TYR | ASP | LEU | GLU | ARG | LEU | ALA | SER | LEU | ||||
| 10 | ASN | PRO | GLU | GLU | LEU | ALA | GLU | LYS | VAL | GLU | ||||
| 11 | GLY | LEU | GLY | GLU | GLU | LEU | ALA | GLU | ARG | VAL | ||||
| 12 | VAL | TYR | ALA | ALA | ARG | GLU | ARG | VAL | GLU | SER | ||||
| 13 | ARG | ARG | LYS | SER | GLY | ARG | GLN | GLU | ARG | SER | ||||
| 14 | GLU | GLU | GLU | TRP | LYS |
Samples:
sample_1: Topoisomerase V repeat FG, [U-100% 13C; U-100% 15N], 575 uM; sodium phosphate 50 mM; sodium chloride 50 mM
sample_2: Topoisomerase V repeat FG, [U-100% 15N], 825 uM; sodium phosphate 50 mM; sodium chloride 50 mM
sample_conditions_1: pH: 6.4; pressure: 1 atm; temperature: 313 K
sample_conditions_2: pH: 6.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N TROSY | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N TROSY | sample_2 | isotropic | sample_conditions_2 |
| 3D HN(CA)N | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN - collection
NMRPipe - processing
TALOS-N - data analysis
CARA - chemical shift assignment
CS-Rosetta - structure solution
NMR spectrometers:
- Bruker AVANCE II 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts