BMRB Entry 51983

Name: Chemical shift assignments for the seventh and eighth (HhH)2 repeats from Topoisomerase V
Published: 2023-08-19

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51983

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Chemical shift assignments for the seventh and eighth (HhH)2 repeats from Topoisomerase V PubMed: 37262333

Deposition date: 2023-05-30 Original release date: 2023-08-19

Authors: Petersen, Mark; Fang, Rebecca; Majumdar, Ananya; Barrick, Doug

Citation: Petersen, Mark; Fang, Rebecca; Majumdar, Ananya; Barrick, Doug. "Stability Islands and the Folding Cooperativity of a Seven-Repeat Array from Topoisomerase V" J. Am. Chem. Soc. 145, 12641-12650 (2023).

Assembly members:
entity_1, polymer, 139 residues, Formula weight is not available

Natural source: Common Name: Methanopyrus kandleri Taxonomy ID: 2320 Superkingdom: Archaea Kingdom: not available Genus/species: Methanopyrus kandleri

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pMal

Entity Sequences (FASTA):
entity_1: GGSWGPEFKFLLNIEGVGPK LAERILEAVDYDLERLASLN PEELAEKVEGLGEELAERVV YAARERVESRRKSGRQERSE EEWKEWLERKVGEGRARRLI EYFGSAGEVGKLVENAEVSK LLEVPGIGDEAVARLVPGY

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	398
15N chemical shifts	132
1H chemical shifts	132

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Topoisomerase V repeats GH	1

Entities:

Entity 1, Topoisomerase V repeats GH 139 residues - Formula weight is not available

1

GLY

SER

TRP

GLY

PRO

GLU

PHE

LYS

PHE

2

LEU

ASN

ILE

GLU

GLY

VAL

GLY

PRO

LYS

3

LEU

ALA

GLU

ARG

ILE

LEU

GLU

ALA

VAL

ASP

4

TYR

ASP

LEU

GLU

ARG

LEU

ALA

SER

LEU

ASN

5

PRO

GLU

LEU

ALA

GLU

LYS

VAL

GLU

GLY

6

LEU

GLY

GLU

LEU

ALA

GLU

ARG

VAL

7

TYR

ALA

ARG

GLU

ARG

VAL

GLU

SER

ARG

8

ARG

LYS

SER

GLY

ARG

GLN

GLU

ARG

SER

GLU

9

GLU

TRP

LYS

GLU

TRP

LEU

GLU

ARG

LYS

10

VAL

GLY

GLU

GLY

ARG

ALA

ARG

LEU

ILE

11

GLU

TYR

PHE

GLY

SER

ALA

GLY

GLU

VAL

GLY

12

LYS

LEU

VAL

GLU

ASN

ALA

GLU

VAL

SER

LYS

13

LEU

GLU

VAL

PRO

GLY

ILE

GLY

ASP

GLU

14

ALA

VAL

ALA

ARG

LEU

VAL

PRO

GLY

TYR

Samples:

sample_1: Topoisomerase V repeat G, [U-100% 13C; U-100% 15N], 500 uM; sodium phosphate 50 mM; sodium chloride 50 mM

sample_2: Topoisomerase V repeat G, [U-100% 15N], 500 uM; sodium phosphate 50 mM; sodium chloride 50 mM

sample_conditions_1: pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
2D 1H-15N TROSY	sample_2	isotropic	sample_conditions_1
3D HN(CA)N	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN - collection

NMRPipe - processing

TALOS-N - data analysis

CARA - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts