BMRB Entry 51996
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR51996
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Title: DNAJB6 J-domain PubMed: 37923706
Deposition date: 2023-06-11 Original release date: 2023-10-30
Authors: Abayev-Avraham, Meital; Rosenzweig, Rina
Citation: Abayev-Avraham, Meital; Salzberg, Yehuda; Gliksberg, Dar; Oren-Suissa, Meital; Rosenzweig, Rina. "DNAJB6 mutants display toxic gain of function through unregulated interaction with Hsp70 chaperones" Nat. Commun. 14, 7066-7066 (2023).
Assembly members:
entity_1, polymer, 72 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-sumo
Entity Sequences (FASTA):
entity_1: MVDYYEVLGVQRHASPEDIK
KAYRKLALKWHPDKNPENKE
EAERKFKQVAEAYEVLSDAK
KRDIYDKYGKEG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 135 |
| 15N chemical shifts | 68 |
| 1H chemical shifts | 68 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DNAJB6 JD | 1 |
Entities:
Entity 1, DNAJB6 JD 72 residues - Formula weight is not available
| 1 | MET | VAL | ASP | TYR | TYR | GLU | VAL | LEU | GLY | VAL | ||||
| 2 | GLN | ARG | HIS | ALA | SER | PRO | GLU | ASP | ILE | LYS | ||||
| 3 | LYS | ALA | TYR | ARG | LYS | LEU | ALA | LEU | LYS | TRP | ||||
| 4 | HIS | PRO | ASP | LYS | ASN | PRO | GLU | ASN | LYS | GLU | ||||
| 5 | GLU | ALA | GLU | ARG | LYS | PHE | LYS | GLN | VAL | ALA | ||||
| 6 | GLU | ALA | TYR | GLU | VAL | LEU | SER | ASP | ALA | LYS | ||||
| 7 | LYS | ARG | ASP | ILE | TYR | ASP | LYS | TYR | GLY | LYS | ||||
| 8 | GLU | GLY |
Samples:
sample_1: DNAJB6 JD, [U-100% 13C; U-100% 15N], 3.5 mM
sample_conditions_1: ionic strength: 50 mM; pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 1000 MHz
- Bruker AVANCE III 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts