BMRB Entry 52012
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52012
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Title: Chemical shifts of Methanosarcina acetivorans mechanosensitive channel of large conductance(Ma-MscL) in native cellular membranes by solid-state NMR PubMed: 38155644
Deposition date: 2023-07-03 Original release date: 2023-07-05
Authors: Zhang, Yan
Citation: Zhang, Yan; Gan, Yuefang; Zhao, Weijing; Zhang, Xuning; Zhao, Yongxiang; Xie, Huayong; Yang, Jun. "Membrane Protein Structures in Native Cellular Membranes Revealed by Solid-State NMR Spectroscopy" JACS Au. 3, 3412-3423 (2023).
Assembly members:
entity_1, polymer, 101 residues, Formula weight is not available
Natural source: Common Name: Methanosarcina acetivorans Taxonomy ID: 2214 Superkingdom: Archaea Kingdom: not available Genus/species: Methanosarcina acetivorans
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
entity_1: MGLFSEFKEFLYEYKVIPLA
IAFIMGIASTALIKSFVDNI
IMPIITPFVPGGGWETATVE
LGPIVISWGAFLGELVNFII
IAFAVFIIAKKVLQEEKVEK
K
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 302 |
| 15N chemical shifts | 73 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MaMscL, subunit 1 | 1 |
| 2 | MaMscL, subunit 2 | 1 |
| 3 | MaMscL, subunit 3 | 1 |
| 4 | MaMscL, subunit 4 | 1 |
| 5 | MaMscL, subunit 5 | 1 |
Entities:
Entity 1, MaMscL, subunit 1 101 residues - Formula weight is not available
| 1 | MET | GLY | LEU | PHE | SER | GLU | PHE | LYS | GLU | PHE | ||||
| 2 | LEU | TYR | GLU | TYR | LYS | VAL | ILE | PRO | LEU | ALA | ||||
| 3 | ILE | ALA | PHE | ILE | MET | GLY | ILE | ALA | SER | THR | ||||
| 4 | ALA | LEU | ILE | LYS | SER | PHE | VAL | ASP | ASN | ILE | ||||
| 5 | ILE | MET | PRO | ILE | ILE | THR | PRO | PHE | VAL | PRO | ||||
| 6 | GLY | GLY | GLY | TRP | GLU | THR | ALA | THR | VAL | GLU | ||||
| 7 | LEU | GLY | PRO | ILE | VAL | ILE | SER | TRP | GLY | ALA | ||||
| 8 | PHE | LEU | GLY | GLU | LEU | VAL | ASN | PHE | ILE | ILE | ||||
| 9 | ILE | ALA | PHE | ALA | VAL | PHE | ILE | ILE | ALA | LYS | ||||
| 10 | LYS | VAL | LEU | GLN | GLU | GLU | LYS | VAL | GLU | LYS | ||||
| 11 | LYS |
Samples:
sample_1: Inner membranes containing MaMscL, [U-100% 13C; U-100% 15N], 76.9% % w/w; H2O 23.1% % w/w
sample_conditions_1: pH: 8.0; pressure: 101.325 kPa; temperature: 277 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NCACX | sample_1 | isotropic | sample_conditions_1 |
| NCOCX | sample_1 | isotropic | sample_conditions_1 |
| CONCA | sample_1 | isotropic | sample_conditions_1 |
Software:
TALOS+ - data analysis
SPARKY - chemical shift assignment
NMR spectrometers:
- Bruker Avance 800 MHz