BMRB Entry 52041
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52041
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Title: AtMed25ACID + DREB2A PubMed: 38238333
Deposition date: 2023-07-15 Original release date: 2023-07-21
Authors: Theisen, Frederik; Prestel, Andreas; Leurs, Yannick; Kragelund, Birthe; Skriver, Karen
Citation: Theisen, Frederik; Prestel, Andreas; Elkjaer, Steffie; Leurs, Yannick; Morffy, Nicholas; Strader, Lucia; O'shea, Charlotte; Teilum, Kaare; Kragelund, Birthe; Skriver, Karen. "Molecular switching in transcription through splicing and proline-isomerization regulates stress responses in plants" Nat. Comm. 15, 592-592 (2024).
Assembly members:
entity_1, polymer, 150 residues, Formula weight is not available
entity_2, polymer, 44 residues, Formula weight is not available
Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX 4t1
Entity Sequences (FASTA):
entity_1: GSNSSAPNMQLSQPSSGAMQ
TSQSKYVKVWEGNLSGQRQG
QPVLITRLEGYRSASASDSL
AANWPPTMQIVRLISQDHMN
NKQYVGKADFLVFRAMSQHG
FLGQLQDKKLCAVIQLPSQT
LLLSVSDKACRLIGMLFPGD
MVVFKPQIPN
entity_2: GQDSLSVADYGWPNDVDQSH
LDSSDMFDVDELLRDLNGDD
VFAG
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 138 |
| 1H chemical shifts | 138 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Med25 | 1 |
| 2 | DREB2A | 2 |
Entities:
Entity 1, Med25 150 residues - Formula weight is not available
Med25ACID 532-680
| 1 | GLY | SER | ASN | SER | SER | ALA | PRO | ASN | MET | GLN | |
| 2 | LEU | SER | GLN | PRO | SER | SER | GLY | ALA | MET | GLN | |
| 3 | THR | SER | GLN | SER | LYS | TYR | VAL | LYS | VAL | TRP | |
| 4 | GLU | GLY | ASN | LEU | SER | GLY | GLN | ARG | GLN | GLY | |
| 5 | GLN | PRO | VAL | LEU | ILE | THR | ARG | LEU | GLU | GLY | |
| 6 | TYR | ARG | SER | ALA | SER | ALA | SER | ASP | SER | LEU | |
| 7 | ALA | ALA | ASN | TRP | PRO | PRO | THR | MET | GLN | ILE | |
| 8 | VAL | ARG | LEU | ILE | SER | GLN | ASP | HIS | MET | ASN | |
| 9 | ASN | LYS | GLN | TYR | VAL | GLY | LYS | ALA | ASP | PHE | |
| 10 | LEU | VAL | PHE | ARG | ALA | MET | SER | GLN | HIS | GLY | |
| 11 | PHE | LEU | GLY | GLN | LEU | GLN | ASP | LYS | LYS | LEU | |
| 12 | CYS | ALA | VAL | ILE | GLN | LEU | PRO | SER | GLN | THR | |
| 13 | LEU | LEU | LEU | SER | VAL | SER | ASP | LYS | ALA | CYS | |
| 14 | ARG | LEU | ILE | GLY | MET | LEU | PHE | PRO | GLY | ASP | |
| 15 | MET | VAL | VAL | PHE | LYS | PRO | GLN | ILE | PRO | ASN |
Entity 2, DREB2A 44 residues - Formula weight is not available
Gly-DREB2A 234-276
| 1 | GLY | GLN | ASP | SER | LEU | SER | VAL | ALA | ASP | TYR | ||||
| 2 | GLY | TRP | PRO | ASN | ASP | VAL | ASP | GLN | SER | HIS | ||||
| 3 | LEU | ASP | SER | SER | ASP | MET | PHE | ASP | VAL | ASP | ||||
| 4 | GLU | LEU | LEU | ARG | ASP | LEU | ASN | GLY | ASP | ASP | ||||
| 5 | VAL | PHE | ALA | GLY |
Samples:
sample_1: Med25ACID, [U-100% 13C; U-100% 15N], 110 uM; DREB2A 234-276 220 uM; Sodium Phosphate buffer 20 mM; NaCl 100 mM; DTT 5 mM
sample_conditions_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 298.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR - chemical shift assignment
qMDD - processing
NMR spectrometers:
- Bruker AVANCE III 600 MHz
- Bruker AVANCE III 750 MHz
- Bruker AVANCE III 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts