BMRB Entry 52071
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52071
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NMR-STAR v3 text file.
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Title: Backbone assignment of Ca2+ bound Arabidopsis thaliana Calmodulin1 PubMed: 37717348
Deposition date: 2023-08-07 Original release date: 2023-09-30
Authors: Pedretti, Marco; Favretto, Filippo; Troilo, Francesca; Giovannoni, Moira; Conter, Carolina; Mattei, Benedetta; Dominici, Paola; Travaglini-Allocatelli, Carlo; Di Matteo, Adele; Astegno, Alessandra
Citation: Pedretti, Marco; Favretto, Filippo; Troilo, Francesca; Giovannoni, Moira; Conter, Carolina; Mattei, Benedetta; Dominici, Paola; Travaglini-Allocatelli, Carlo; Di Matteo, Adele; Astegno, Alessandra. "Role of myristoylation in modulating PCaP1 interaction with calmodulin" Plant Physiol. Biochem. 203, 108003-108003 (2023).
Assembly members:
entity_1, polymer, 149 residues, Formula weight is not available
entity_CA, non-polymer, 40.078 Da.
Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET12b
Entity Sequences (FASTA):
entity_1: MADQLTDEQISEFKEAFSLF
DKDGDGCITTKELGTVMRSL
GQNPTEAELQDMINEVDADG
NGTIDFPEFLNLMAKKMKDT
DSEEELKEAFRVFDKDQNGF
ISAAELRHVMTNLGEKLTDE
EVEEMIREADVDGDGQINYE
EFVKIMMAK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 427 |
| 15N chemical shifts | 144 |
| 1H chemical shifts | 144 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | AtCaM1 calcium bound | 1 |
| 2 | calcium ion, 1 | 2 |
| 3 | calcium ion, 2 | 2 |
| 4 | calcium ion, 3 | 2 |
| 5 | calcium ion, 4 | 2 |
Entities:
Entity 1, AtCaM1 calcium bound 149 residues - Formula weight is not available
| 1 | MET | ALA | ASP | GLN | LEU | THR | ASP | GLU | GLN | ILE | ||||
| 2 | SER | GLU | PHE | LYS | GLU | ALA | PHE | SER | LEU | PHE | ||||
| 3 | ASP | LYS | ASP | GLY | ASP | GLY | CYS | ILE | THR | THR | ||||
| 4 | LYS | GLU | LEU | GLY | THR | VAL | MET | ARG | SER | LEU | ||||
| 5 | GLY | GLN | ASN | PRO | THR | GLU | ALA | GLU | LEU | GLN | ||||
| 6 | ASP | MET | ILE | ASN | GLU | VAL | ASP | ALA | ASP | GLY | ||||
| 7 | ASN | GLY | THR | ILE | ASP | PHE | PRO | GLU | PHE | LEU | ||||
| 8 | ASN | LEU | MET | ALA | LYS | LYS | MET | LYS | ASP | THR | ||||
| 9 | ASP | SER | GLU | GLU | GLU | LEU | LYS | GLU | ALA | PHE | ||||
| 10 | ARG | VAL | PHE | ASP | LYS | ASP | GLN | ASN | GLY | PHE | ||||
| 11 | ILE | SER | ALA | ALA | GLU | LEU | ARG | HIS | VAL | MET | ||||
| 12 | THR | ASN | LEU | GLY | GLU | LYS | LEU | THR | ASP | GLU | ||||
| 13 | GLU | VAL | GLU | GLU | MET | ILE | ARG | GLU | ALA | ASP | ||||
| 14 | VAL | ASP | GLY | ASP | GLY | GLN | ILE | ASN | TYR | GLU | ||||
| 15 | GLU | PHE | VAL | LYS | ILE | MET | MET | ALA | LYS |
Entity 2, calcium ion, 1 - Ca - 40.078 Da.
| 1 | CA |
Samples:
sample_1: AtCaM1 Ca2+, [U-100% 13C; U-100% 15N], 1.6 mM; KCl 50 mM; TRIS 50 mM; DTT 0.5 mM
sample_conditions_1: ionic strength: 0.05 M; pH: 7.5; pressure: 1 atm; temperature: 298.15 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR v2.5.2 - chemical shift assignment, peak picking
NMRPipe v9.9 - processing
NMR spectrometers:
- Bruker AVANCE III 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts