BMRB Entry 52104
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52104
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Title: Solid State NMR chemical shift of Ost4V23D PubMed: 38421550
Deposition date: 2023-08-26 Original release date: 2024-03-09
Authors: Chaudhary, Bharat; Struppe, Jochem; Moktan, Hem; Zoetewey, David; zhou, Donghua; Mohanty, Smita
Citation: Chaudhary, Bharat; Struppe, Jochem; Moktan, Hem; Zoetewey, David; zhou, Donghua; Mohanty, Smita. "Reconstitution and resonance assignments of yeast OST subunit Ost4 and its critical mutant Ost4V23D in liposomes by solid-state NMR" J. Biomol. NMR ., .-. (2024).
Assembly members:
entity_1, polymer, 39 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: GEV2
Entity Sequences (FASTA):
entity_1: MISDEQLNSLAITFGIVMMT
LIDIYHAVDSTMSPKNRLE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 131 |
| 15N chemical shifts | 35 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Ost4V23D | 1 |
Entities:
Entity 1, Ost4V23D 39 residues - Formula weight is not available
| 1 | MET | ILE | SER | ASP | GLU | GLN | LEU | ASN | SER | LEU | ||||
| 2 | ALA | ILE | THR | PHE | GLY | ILE | VAL | MET | MET | THR | ||||
| 3 | LEU | ILE | ASP | ILE | TYR | HIS | ALA | VAL | ASP | SER | ||||
| 4 | THR | MET | SER | PRO | LYS | ASN | ARG | LEU | GLU |
Samples:
sample_1: Ost4V23D, [U-100% 13C; U-100% 15N], 14 mg
sample_conditions_1: pH: 6.5; temperature: 230 to 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 13C-13C DARR | sample_1 | isotropic | sample_conditions_1 |
| 3D NCOCX | sample_1 | isotropic | sample_conditions_1 |
| 3D CANcoCX | sample_1 | isotropic | sample_conditions_1 |
| 3D NCACX | sample_1 | isotropic | sample_conditions_1 |
| 2D CHHC | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR v2.1 - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 700 MHz