BMRB Entry 52130
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52130
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Title: Backbone assignment of C-teminus of yeast Oxa1 PubMed: 37834108
Deposition date: 2023-09-17 Original release date: 2023-11-15
Authors: Liu, Yong; Yang, Jing; Ruan, Maosen; Zhang, Huiqin; Wang, Junfeng; Li, Yunyan
Citation: Liu, Yong; Yang, Jing; Ruan, Maosen; Zhang, Huiqin; Wang, Junfeng; Li, Yunyan. "NMR-Based Characterization of the Interaction between Yeast Oxa1-CTD and Ribosomes" Int. J. Mol. Sci . 24, 14657-14657 (2023).
Assembly members:
entity_1, polymer, 104 residues, Formula weight is not available
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET14b
Entity Sequences (FASTA):
entity_1: MGSSHHHHHHSSGLVPRGSH
MWVRSKLKITEVAKPRTPIA
GASPTENMGIFQSLKHNIQK
ARDQAERRQLMQDNEKKLQE
SFKEKRQNSKIKIVHKSNFI
NNKK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 521 |
| 15N chemical shifts | 151 |
| 1H chemical shifts | 872 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Oxa1-CTD | 1 |
Entities:
Entity 1, Oxa1-CTD 104 residues - Formula weight is not available
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 3 | MET | TRP | VAL | ARG | SER | LYS | LEU | LYS | ILE | THR | ||||
| 4 | GLU | VAL | ALA | LYS | PRO | ARG | THR | PRO | ILE | ALA | ||||
| 5 | GLY | ALA | SER | PRO | THR | GLU | ASN | MET | GLY | ILE | ||||
| 6 | PHE | GLN | SER | LEU | LYS | HIS | ASN | ILE | GLN | LYS | ||||
| 7 | ALA | ARG | ASP | GLN | ALA | GLU | ARG | ARG | GLN | LEU | ||||
| 8 | MET | GLN | ASP | ASN | GLU | LYS | LYS | LEU | GLN | GLU | ||||
| 9 | SER | PHE | LYS | GLU | LYS | ARG | GLN | ASN | SER | LYS | ||||
| 10 | ILE | LYS | ILE | VAL | HIS | LYS | SER | ASN | PHE | ILE | ||||
| 11 | ASN | ASN | LYS | LYS |
Samples:
sample_1: Oxa1-CTD, [U-95% 15N], 0.045 mM; Oxa1-CTD, [U-13C; U-15N], 0.5 mM; HEPES 20 mM; NaCl 75 mM; MgCl2 20 mM; b-mercaptoethanol 5 mM
sample_conditions_1: ionic strength: 0.1 M; pH: 7.2; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D CBCACONH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 850 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts