BMRB Entry 52137
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52137
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Title: Backbone assignment of ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1) PubMed: 38185323
Deposition date: 2023-09-19 Original release date: 2024-01-09
Authors: Chiang, Tsung-Sheng; Lai, Chih-Hsuan; Hsu, Shang-Te Danny
Citation: Kenny, Sebastian; Lai, Chih-Hsuan; Chiang, Tsung-Sheng; Brown, Kwame; Hewitt, Chad; Krabill, Aaron; Chang, Hao-Ting; Wang, Yong-Sheng; Flaherty, Daniel; Danny Hsu, Shang-Te; Das, Chittaranjan. "Altered protein dynamics and a more reactive catalytic cysteine in a neurodegeneration-associated UCHL1 mutant" J. Mol. Biol. 436, 168438-168438 (2024).
Assembly members:
entity_1, polymer, 231 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET23a
Entity Sequences (FASTA):
entity_1: MQLKPMEINPEMLNKVLSRL
GVAGQWRFVDVLGLEEESLG
SVPAPACALLLLFPLTAQHE
NFRKKQIEELKGQEVSPKVY
FMKQTIGNSCGTIGLIHAVA
NNQDKLGFEDGSVLKQFLSE
TEKMSPEDRAKCFEKNEAIQ
AAHDAVAQEGQCRVDDKVNF
HFILFNNVDGHLYELDGRMP
FPVNHGASSEDTLLKDAAKV
CREFTEREQGEVRFSAVALC
KAALEHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 632 |
| 15N chemical shifts | 209 |
| 1H chemical shifts | 209 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | UCHL1 | 1 |
Entities:
Entity 1, UCHL1 231 residues - Formula weight is not available
| 1 | MET | GLN | LEU | LYS | PRO | MET | GLU | ILE | ASN | PRO | ||||
| 2 | GLU | MET | LEU | ASN | LYS | VAL | LEU | SER | ARG | LEU | ||||
| 3 | GLY | VAL | ALA | GLY | GLN | TRP | ARG | PHE | VAL | ASP | ||||
| 4 | VAL | LEU | GLY | LEU | GLU | GLU | GLU | SER | LEU | GLY | ||||
| 5 | SER | VAL | PRO | ALA | PRO | ALA | CYS | ALA | LEU | LEU | ||||
| 6 | LEU | LEU | PHE | PRO | LEU | THR | ALA | GLN | HIS | GLU | ||||
| 7 | ASN | PHE | ARG | LYS | LYS | GLN | ILE | GLU | GLU | LEU | ||||
| 8 | LYS | GLY | GLN | GLU | VAL | SER | PRO | LYS | VAL | TYR | ||||
| 9 | PHE | MET | LYS | GLN | THR | ILE | GLY | ASN | SER | CYS | ||||
| 10 | GLY | THR | ILE | GLY | LEU | ILE | HIS | ALA | VAL | ALA | ||||
| 11 | ASN | ASN | GLN | ASP | LYS | LEU | GLY | PHE | GLU | ASP | ||||
| 12 | GLY | SER | VAL | LEU | LYS | GLN | PHE | LEU | SER | GLU | ||||
| 13 | THR | GLU | LYS | MET | SER | PRO | GLU | ASP | ARG | ALA | ||||
| 14 | LYS | CYS | PHE | GLU | LYS | ASN | GLU | ALA | ILE | GLN | ||||
| 15 | ALA | ALA | HIS | ASP | ALA | VAL | ALA | GLN | GLU | GLY | ||||
| 16 | GLN | CYS | ARG | VAL | ASP | ASP | LYS | VAL | ASN | PHE | ||||
| 17 | HIS | PHE | ILE | LEU | PHE | ASN | ASN | VAL | ASP | GLY | ||||
| 18 | HIS | LEU | TYR | GLU | LEU | ASP | GLY | ARG | MET | PRO | ||||
| 19 | PHE | PRO | VAL | ASN | HIS | GLY | ALA | SER | SER | GLU | ||||
| 20 | ASP | THR | LEU | LEU | LYS | ASP | ALA | ALA | LYS | VAL | ||||
| 21 | CYS | ARG | GLU | PHE | THR | GLU | ARG | GLU | GLN | GLY | ||||
| 22 | GLU | VAL | ARG | PHE | SER | ALA | VAL | ALA | LEU | CYS | ||||
| 23 | LYS | ALA | ALA | LEU | GLU | HIS | HIS | HIS | HIS | HIS | ||||
| 24 | HIS |
Samples:
sample_1: ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1), [U-13C; U-15N], 1 mM; EDTA 0.5 mM; TCEP 1 mM; NaN3 0.02%
sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCACONH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe - processing
NMRFAM-SPARKY - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts