BMRB Entry 52171
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52171
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Sipa assignment
Deposition date: 2023-10-13 Original release date: 2024-02-26
Authors: Neira, Jose Luis
Citation: Neira, Jose Luis. "Structure and dynamics of the regulatory factor SipA" .
Assembly members:
entity_1, polymer, 78 residues, Formula weight is not available
Natural source: Common Name: Synechococcus elongatus Taxonomy ID: 32046 Superkingdom: Bacteria Kingdom: not available Genus/species: Synechococcus elongatus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pPROEX-HTa
Entity Sequences (FASTA):
entity_1: MDFAIGDRVRLAAQPPYLKS
ADPLPMLRPPDLLAVGEQGT
ITGLRPGGYWVVLFDRGSFL
LDTQFLSKVESGASSSEG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 210 |
| 15N chemical shifts | 77 |
| 1H chemical shifts | 506 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Regulatory factor SipA | 1 |
Entities:
Entity 1, Regulatory factor SipA 78 residues - Formula weight is not available
| 1 | MET | ASP | PHE | ALA | ILE | GLY | ASP | ARG | VAL | ARG | ||||
| 2 | LEU | ALA | ALA | GLN | PRO | PRO | TYR | LEU | LYS | SER | ||||
| 3 | ALA | ASP | PRO | LEU | PRO | MET | LEU | ARG | PRO | PRO | ||||
| 4 | ASP | LEU | LEU | ALA | VAL | GLY | GLU | GLN | GLY | THR | ||||
| 5 | ILE | THR | GLY | LEU | ARG | PRO | GLY | GLY | TYR | TRP | ||||
| 6 | VAL | VAL | LEU | PHE | ASP | ARG | GLY | SER | PHE | LEU | ||||
| 7 | LEU | ASP | THR | GLN | PHE | LEU | SER | LYS | VAL | GLU | ||||
| 8 | SER | GLY | ALA | SER | SER | SER | GLU | GLY |
Samples:
sample_1: Regulatory factor SipA 2.5 mM
sample_2: Regulatory factor SipA, [U-100% 13C; U-100% 15N], 2.5 mM
sample_conditions_1: ionic strength: 0.050 M; pH: 5.5; pressure: 1 atm; temperature: 298 K
sample_conditions_2: ionic strength: 0.050 M; pH: 5.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_2 | isotropic | sample_conditions_2 |
| 3D HNCO | sample_2 | isotropic | sample_conditions_2 |
| 3D CBCACONH | sample_2 | isotropic | sample_conditions_2 |
| 3D HNCA | sample_2 | isotropic | sample_conditions_2 |
| 3D HNCACB | sample_2 | isotropic | sample_conditions_2 |
| 3D H(CCO)NH | sample_2 | isotropic | sample_conditions_2 |
| 3D 1H-15N NOESY | sample_2 | isotropic | sample_conditions_2 |
Software:
SPARKY - chemical shift assignment
NMR spectrometers:
- Bruker Avance 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts