BMRB Entry 52197
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52197
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Title: Partial solid-state NMR 13C, 15N resonance assignments of the two conformations of Bj-SemiSWEET in native celular membranes PubMed: 38155644
Deposition date: 2023-10-31 Original release date: 2024-01-04
Authors: Xie, Huayong; Gan, yuefang; Yang, jun
Citation: Zhang, Yan; Gan, Yuefang; Zhao, Weijing; Zhang, Xuning; Zhao, Yongxiang; Xie, Huayong; Yang, Jun. "Membrane Protein Structures in Native Cellular Membranes Revealed by Solid-State NMR Spectroscopy" JACS Au. 3, 3412-3423 (2023).
Assembly members:
entity_1, polymer, 86 residues, Formula weight is not available
Natural source: Common Name: Bradyrhizobium Taxonomy ID: 374 Superkingdom: Bacteria Kingdom: not available Genus/species: Bradyrhizobium not available
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pSD005
Entity Sequences (FASTA):
entity_1: MDPFLIKLIGFAAATCTTVA
YAPQFIKVLKTRSARDISLG
MFLVMVLGLALWLIYGLLSG
DAPLIASNAVTMLLAGGILV
MKLRYG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 620 |
| 15N chemical shifts | 170 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Bj-SemiSWEET, conformer A | 1 |
| 2 | Bj-SemiSWEET, conformer B | 1 |
Entities:
Entity 1, Bj-SemiSWEET, conformer A 86 residues - Formula weight is not available
NCBI Reference Sequence: WP_011089200.1
| 1 | MET | ASP | PRO | PHE | LEU | ILE | LYS | LEU | ILE | GLY | ||||
| 2 | PHE | ALA | ALA | ALA | THR | CYS | THR | THR | VAL | ALA | ||||
| 3 | TYR | ALA | PRO | GLN | PHE | ILE | LYS | VAL | LEU | LYS | ||||
| 4 | THR | ARG | SER | ALA | ARG | ASP | ILE | SER | LEU | GLY | ||||
| 5 | MET | PHE | LEU | VAL | MET | VAL | LEU | GLY | LEU | ALA | ||||
| 6 | LEU | TRP | LEU | ILE | TYR | GLY | LEU | LEU | SER | GLY | ||||
| 7 | ASP | ALA | PRO | LEU | ILE | ALA | SER | ASN | ALA | VAL | ||||
| 8 | THR | MET | LEU | LEU | ALA | GLY | GLY | ILE | LEU | VAL | ||||
| 9 | MET | LYS | LEU | ARG | TYR | GLY |
Samples:
sample_1: Bj-SemiSWEET, [U-13C; U-15N], 0.7 w/w
sample_conditions_1: pH: 7.5; pressure: 1 atm; temperature: 277 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NCACX | sample_1 | isotropic | sample_conditions_1 |
| NCOCX | sample_1 | isotropic | sample_conditions_1 |
| CONCA | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe - data analysis
NMR spectrometers:
- Bruker AVANCE III 800 MHz
Related Database Links:
| NCBI | WP_011089200 |