BMRB Entry 52266
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52266
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Title: Backbone chemical shift assignment of HoxA9 protein
Deposition date: 2024-01-09 Original release date: 2024-02-08
Authors: Saibo, Nikita; De, Soumya
Citation: Saibo, Nikita; Maiti, Snigdha; Boral, Soumendu; Banerjee, Puja; Kushwaha, Tushar; Inampudi, Krishna; Goswami, Ritobrata; De, Soumya. "The intrinsically disordered transactivation region of HOXA9 regulates its function by auto-inhibition of its DNA-binding activity" IJBM ., .-..
Assembly members:
entity_1, polymer, 87 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
entity_1: RRRRRRRRNNPAANWLHARS
TRKKRCPYTKHQTLELEKEF
LFNMYLTRDRRYEVARLLNL
TERQVKIWFQNRRMKMKKIN
KDRAKDE
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 142 |
15N chemical shifts | 75 |
1H chemical shifts | 76 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | HoxA9 Monomer | 1 |
Entities:
Entity 1, HoxA9 Monomer 87 residues - Formula weight is not available
Residue 194-266
1 | ARG | ARG | ARG | ARG | ARG | ARG | ARG | ARG | ASN | ASN | ||||
2 | PRO | ALA | ALA | ASN | TRP | LEU | HIS | ALA | ARG | SER | ||||
3 | THR | ARG | LYS | LYS | ARG | CYS | PRO | TYR | THR | LYS | ||||
4 | HIS | GLN | THR | LEU | GLU | LEU | GLU | LYS | GLU | PHE | ||||
5 | LEU | PHE | ASN | MET | TYR | LEU | THR | ARG | ASP | ARG | ||||
6 | ARG | TYR | GLU | VAL | ALA | ARG | LEU | LEU | ASN | LEU | ||||
7 | THR | GLU | ARG | GLN | VAL | LYS | ILE | TRP | PHE | GLN | ||||
8 | ASN | ARG | ARG | MET | LYS | MET | LYS | LYS | ILE | ASN | ||||
9 | LYS | ASP | ARG | ALA | LYS | ASP | GLU |
Samples:
sample_1: HoxA9 Monomer, [U-100% 13C], 0.3 mM; sodium phosphate 20 mM; sodium chloride 50 mM
sample_conditions_1: ionic strength: 50 mM; pH: 5.5; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D CBCACONH | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts