BMRB Entry 52316
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52316
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Title: Vcom2 PubMed: 38747744
Deposition date: 2024-02-08 Original release date: 2024-07-22
Authors: Khatibi, Negin; Huang, Yen-Hua; Wang, Conan; Durek, Thomas; Gilding, Edward; Craik, David
Citation: Khatibi, Negin; Huang, Yen-Hua; Wang, Conan; Durek, Thomas; Gilding, Edward; Craik, David. "Isolation and Characterization of Insecticidal Cyclotides from Viola communis" J. Nat. Prod. ., .-. (2024).
Assembly members:
entity_1, polymer, 30 residues, Formula weight is not available
Natural source: Common Name: Violaceae Taxonomy ID: 24921 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: not available not available
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
entity_1: GAPVCGETCFGGTCNTPGCT
CDPWPVCSKN
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 69 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Vcom2 | 1 |
Entities:
Entity 1, Vcom2 30 residues - Formula weight is not available
| 1 | GLY | ALA | PRO | VAL | CYS | GLY | GLU | THR | CYS | PHE | |
| 2 | GLY | GLY | THR | CYS | ASN | THR | PRO | GLY | CYS | THR | |
| 3 | CYS | ASP | PRO | TRP | PRO | VAL | CYS | SER | LYS | ASN |
Samples:
sample_1: Vcom2 0.7 mg
sample_conditions_1: ionic strength: 0 M; pH: 3.6; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN - collection
NMR spectrometers:
- Bruker ARX 600 MHz