BMRB Entry 52340
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52340
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Title: 4E-tau(297-407) in vitro AD C-shaped PubMed: 38679331
Deposition date: 2024-02-26 Original release date: 2024-07-22
Authors: Duan, Pu; El Mammeri, Nadia; Hong, Mei
Citation: Duan, Pu; El Mammeri, Nadia; Hong, Mei. "Milligram-scale assembly and NMR fingerprint of tau fibrils adopting the Alzheimer's disease fold" J. Biol. Chem. 300, 107326-107326 (2024).
Assembly members:
entity_1, polymer, 112 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli BL21(DE3) Vector: pET-28a
Entity Sequences (FASTA):
entity_1: MIKHVPGGGSVQIVYKPVDL
SKVTSKCGSLGNIHHKPGGG
QVEVKSEKLDFKDRVQSKIG
SLDNITHVPGGGNKKIETHK
LTFRENAKAKTDHGAEIVYK
EPVVEGDEEPRH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 225 |
| 15N chemical shifts | 55 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | 4E-tau(297-407) | 1 |
Entities:
Entity 1, 4E-tau(297-407) 112 residues - Formula weight is not available
| 1 | MET | ILE | LYS | HIS | VAL | PRO | GLY | GLY | GLY | SER | ||||
| 2 | VAL | GLN | ILE | VAL | TYR | LYS | PRO | VAL | ASP | LEU | ||||
| 3 | SER | LYS | VAL | THR | SER | LYS | CYS | GLY | SER | LEU | ||||
| 4 | GLY | ASN | ILE | HIS | HIS | LYS | PRO | GLY | GLY | GLY | ||||
| 5 | GLN | VAL | GLU | VAL | LYS | SER | GLU | LYS | LEU | ASP | ||||
| 6 | PHE | LYS | ASP | ARG | VAL | GLN | SER | LYS | ILE | GLY | ||||
| 7 | SER | LEU | ASP | ASN | ILE | THR | HIS | VAL | PRO | GLY | ||||
| 8 | GLY | GLY | ASN | LYS | LYS | ILE | GLU | THR | HIS | LYS | ||||
| 9 | LEU | THR | PHE | ARG | GLU | ASN | ALA | LYS | ALA | LYS | ||||
| 10 | THR | ASP | HIS | GLY | ALA | GLU | ILE | VAL | TYR | LYS | ||||
| 11 | GLU | PRO | VAL | VAL | GLU | GLY | ASP | GLU | GLU | PRO | ||||
| 12 | ARG | HIS |
Samples:
sample_1: 4E-Tau (297-407), [U-100% 13C; U-100% 15N], 50 % w/w; potassium phosphate buffer 10 mM; magnesium chloride 100 mM
sample_conditions_1: ionic strength: 0.31 M; pH: 7.5; pressure: 1 atm; temperature: 280 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 13C-detected NCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D NCACX | sample_1 | isotropic | sample_conditions_1 |
| 3D NCOCX | sample_1 | isotropic | sample_conditions_1 |
| 3D CONCA | sample_1 | isotropic | sample_conditions_1 |
| 2D 13C-detected CORD | sample_1 | isotropic | sample_conditions_1 |
| 2D 13C-detected NCA | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v4.3 - processing
CcpNmr Analysis v2.4.2 - chemical shift assignment, peak picking
NMR spectrometers:
- Bruker Avance 800 MHz