BMRB Entry 52343
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52343
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Title: Ubiquitin L50A mutant PubMed: 38663546
Deposition date: 2024-03-03 Original release date: 2024-07-22
Authors: Masoumzadeh, Elahe; Ying, Jinfa; Baber, James; Anfinrud, Philip; Bax, Ad
Citation: Masoumzadeh, Elahe; Ying, Jinfa; Baber, James; Anfinrud, Philip; Bax, Ad. "Proline Peptide Bond Isomerization in Ubiquitin Under Folding and Denaturing Conditions by Pressure-Jump NMR" J. Mol. Biol. 436, 168587-168587 (2024).
Assembly members:
entity_1, polymer, 76 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET22
Entity Sequences (FASTA):
entity_1: MQIFVKTLTGKTITLEVEPS
DTIENVKAKIQDKEGIPPDQ
QRLIFAGKQAEDGRTLSDYN
IQKESTLHLVLRLRGG
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 72 |
15N chemical shifts | 70 |
1H chemical shifts | 70 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Ubq L50A | 1 |
Entities:
Entity 1, Ubq L50A 76 residues - Formula weight is not available
1 | MET | GLN | ILE | PHE | VAL | LYS | THR | LEU | THR | GLY | ||||
2 | LYS | THR | ILE | THR | LEU | GLU | VAL | GLU | PRO | SER | ||||
3 | ASP | THR | ILE | GLU | ASN | VAL | LYS | ALA | LYS | ILE | ||||
4 | GLN | ASP | LYS | GLU | GLY | ILE | PRO | PRO | ASP | GLN | ||||
5 | GLN | ARG | LEU | ILE | PHE | ALA | GLY | LYS | GLN | ALA | ||||
6 | GLU | ASP | GLY | ARG | THR | LEU | SER | ASP | TYR | ASN | ||||
7 | ILE | GLN | LYS | GLU | SER | THR | LEU | HIS | LEU | VAL | ||||
8 | LEU | ARG | LEU | ARG | GLY | GLY |
Samples:
sample_1: Ubiquitin L50A mutant, [U-2H; U-15N], 400 uM; sodium phosphate 20 mM; EDTA 1 mM; benzamidine 1 mM
sample_conditions_1: ionic strength: 20 mM; pH: 6.4; pressure: 1 atm; temperature: 278 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N TROSY | sample_1 | isotropic | sample_conditions_1 |
Software:
ccpn analysis - chemical shift assignment
NMRPipe - data analysis
SMILE - data analysis
NMR spectrometers:
- Bruker AVANCE III HD 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts