BMRB Entry 52363
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52363
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: GeoRec1 backbone assignments PubMed: 38746279
Deposition date: 2024-03-25 Original release date: 2024-07-22
Authors: Belato, Helen
Citation: Belato, Helen; Knight, Alexa; D'Ordine, Alexandra; Fan, Zhiqiang; Luo, Jinping; Jogl, Gerwald; Lisi, George. "Atomistic Tuning of the GeoCas9 Recognition Lobe Modulates Allosteric Motions and Guide RNA Interactions" bioRxiv ., .-. (2024).
Assembly members:
entity_1, polymer, 136 residues, Formula weight is not available
Natural source: Common Name: Geobacillus stearothermophilus Taxonomy ID: 1422 Superkingdom: Bacteria Kingdom: not available Genus/species: Geobacillus stearothermophilus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
entity_1: EEKHEIDVWQLRVEALDRKL
NNDELARVLLHLAKRRGFKS
NRKSERSNKENSTMLKHIEE
NRAILSSYRTVGEMIVKDPK
FALHKRNKGENYTNTIARDD
LEREIRLIFSKQREFGNMSC
TEEFENEYITIWASQR
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 266 |
15N chemical shifts | 98 |
1H chemical shifts | 98 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | GeoRec1 | 1 |
Entities:
Entity 1, GeoRec1 136 residues - Formula weight is not available
1 | GLU | GLU | LYS | HIS | GLU | ILE | ASP | VAL | TRP | GLN | ||||
2 | LEU | ARG | VAL | GLU | ALA | LEU | ASP | ARG | LYS | LEU | ||||
3 | ASN | ASN | ASP | GLU | LEU | ALA | ARG | VAL | LEU | LEU | ||||
4 | HIS | LEU | ALA | LYS | ARG | ARG | GLY | PHE | LYS | SER | ||||
5 | ASN | ARG | LYS | SER | GLU | ARG | SER | ASN | LYS | GLU | ||||
6 | ASN | SER | THR | MET | LEU | LYS | HIS | ILE | GLU | GLU | ||||
7 | ASN | ARG | ALA | ILE | LEU | SER | SER | TYR | ARG | THR | ||||
8 | VAL | GLY | GLU | MET | ILE | VAL | LYS | ASP | PRO | LYS | ||||
9 | PHE | ALA | LEU | HIS | LYS | ARG | ASN | LYS | GLY | GLU | ||||
10 | ASN | TYR | THR | ASN | THR | ILE | ALA | ARG | ASP | ASP | ||||
11 | LEU | GLU | ARG | GLU | ILE | ARG | LEU | ILE | PHE | SER | ||||
12 | LYS | GLN | ARG | GLU | PHE | GLY | ASN | MET | SER | CYS | ||||
13 | THR | GLU | GLU | PHE | GLU | ASN | GLU | TYR | ILE | THR | ||||
14 | ILE | TRP | ALA | SER | GLN | ARG |
Samples:
sample_1: GeoRec1 domain of GeoCas9, [U-100% 13C; U-100% 15N], 500 uM; sodium phosphate 20 mM; potassium chloride 80 mM; DTT 1 mM; EDTA 1 mM
sample_conditions_1: pH: 7.4; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CACB | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
Software:
PINE - chemical shift assignment
CARA - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts