BMRB Entry 52371

Name: 1H, 13C and 15N backbone resonance assignment of Cel45A from Phanerochaete chrysosporium
Published: 2024-07-19

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52371

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: 1H, 13C and 15N backbone resonance assignment of Cel45A from Phanerochaete chrysosporium PubMed: 38888713

Deposition date: 2024-03-27 Original release date: 2024-07-19

Authors: Okmane, Laura; Sandgren, Mats; Stahlberg, Jerry; Nestor, Gustav

Citation: Okmane, Laura; Sandgren, Mats; Stahlberg, Jerry; Nestor, Gustav. "1H, 13C and 15N backbone resonance assignment of Cel45A from Phanerochaete chrysosporium" Biomol. NMR Assignments ., .-. (2024).

Assembly members:
entity_1, polymer, 180 residues, Formula weight is not available

Natural source: Common Name: Phanerochaete chrysosporium Taxonomy ID: 2822231 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Phanerochaete chrysosporium

Experimental source: Production method: recombinant technology Host organism: Pichia pastoris Vector: pPICZa

Entity Sequences (FASTA):
entity_1: ATGGYVQQATGQASFTMYSG CGSPACGKAASGFTAAINQL AFGSAPGLGAGDACGRCFAL TGNHDPYSPNYTGPFGQTIV VKVTDLCPVQGNQEFCGQTT SNPTNQHGMPFHFDICEDTG GSAKFFPSGHGALTGTFTEV SCSQWSGSDGGQLWNGACLS GETAPNWPSTACGNKGTAPS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	488
15N chemical shifts	166
1H chemical shifts	555

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PcCel45A	1

Entities:

Entity 1, PcCel45A 180 residues - Formula weight is not available

1	ALA	THR	GLY	GLY	TYR	VAL	GLN	GLN	ALA	THR
2	GLY	GLN	ALA	SER	PHE	THR	MET	TYR	SER	GLY
3	CYS	GLY	SER	PRO	ALA	CYS	GLY	LYS	ALA	ALA
4	SER	GLY	PHE	THR	ALA	ALA	ILE	ASN	GLN	LEU
5	ALA	PHE	GLY	SER	ALA	PRO	GLY	LEU	GLY	ALA
6	GLY	ASP	ALA	CYS	GLY	ARG	CYS	PHE	ALA	LEU
7	THR	GLY	ASN	HIS	ASP	PRO	TYR	SER	PRO	ASN
8	TYR	THR	GLY	PRO	PHE	GLY	GLN	THR	ILE	VAL
9	VAL	LYS	VAL	THR	ASP	LEU	CYS	PRO	VAL	GLN
10	GLY	ASN	GLN	GLU	PHE	CYS	GLY	GLN	THR	THR
11	SER	ASN	PRO	THR	ASN	GLN	HIS	GLY	MET	PRO
12	PHE	HIS	PHE	ASP	ILE	CYS	GLU	ASP	THR	GLY
13	GLY	SER	ALA	LYS	PHE	PHE	PRO	SER	GLY	HIS
14	GLY	ALA	LEU	THR	GLY	THR	PHE	THR	GLU	VAL
15	SER	CYS	SER	GLN	TRP	SER	GLY	SER	ASP	GLY
16	GLY	GLN	LEU	TRP	ASN	GLY	ALA	CYS	LEU	SER
17	GLY	GLU	THR	ALA	PRO	ASN	TRP	PRO	SER	THR
18	ALA	CYS	GLY	ASN	LYS	GLY	THR	ALA	PRO	SER

Samples:

sample_1: PcCel45A, [U-70% 13C; U-95% 15N], 450 uM; D2O, [U-99% 2H], 10%; potassium phosphate 50 mM; sodium azide 0.03%

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v4.1.4 - collection, processing

CcpNMR - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

Bruker AVANCE III 600 MHz MHz

Biological Magnetic Resonance Data Bank