BMRB Entry 52371
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52371
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Title: 1H, 13C and 15N backbone resonance assignment of Cel45A from Phanerochaete chrysosporium PubMed: 38888713
Deposition date: 2024-03-27 Original release date: 2024-07-19
Authors: Okmane, Laura; Sandgren, Mats; Stahlberg, Jerry; Nestor, Gustav
Citation: Okmane, Laura; Sandgren, Mats; Stahlberg, Jerry; Nestor, Gustav. "1H, 13C and 15N backbone resonance assignment of Cel45A from Phanerochaete chrysosporium" Biomol. NMR Assignments ., .-. (2024).
Assembly members:
entity_1, polymer, 180 residues, Formula weight is not available
Natural source: Common Name: Phanerochaete chrysosporium Taxonomy ID: 2822231 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Phanerochaete chrysosporium
Experimental source: Production method: recombinant technology Host organism: Pichia pastoris Vector: pPICZa
Entity Sequences (FASTA):
entity_1: ATGGYVQQATGQASFTMYSG
CGSPACGKAASGFTAAINQL
AFGSAPGLGAGDACGRCFAL
TGNHDPYSPNYTGPFGQTIV
VKVTDLCPVQGNQEFCGQTT
SNPTNQHGMPFHFDICEDTG
GSAKFFPSGHGALTGTFTEV
SCSQWSGSDGGQLWNGACLS
GETAPNWPSTACGNKGTAPS
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 488 |
15N chemical shifts | 166 |
1H chemical shifts | 555 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | PcCel45A | 1 |
Entities:
Entity 1, PcCel45A 180 residues - Formula weight is not available
1 | ALA | THR | GLY | GLY | TYR | VAL | GLN | GLN | ALA | THR | |
2 | GLY | GLN | ALA | SER | PHE | THR | MET | TYR | SER | GLY | |
3 | CYS | GLY | SER | PRO | ALA | CYS | GLY | LYS | ALA | ALA | |
4 | SER | GLY | PHE | THR | ALA | ALA | ILE | ASN | GLN | LEU | |
5 | ALA | PHE | GLY | SER | ALA | PRO | GLY | LEU | GLY | ALA | |
6 | GLY | ASP | ALA | CYS | GLY | ARG | CYS | PHE | ALA | LEU | |
7 | THR | GLY | ASN | HIS | ASP | PRO | TYR | SER | PRO | ASN | |
8 | TYR | THR | GLY | PRO | PHE | GLY | GLN | THR | ILE | VAL | |
9 | VAL | LYS | VAL | THR | ASP | LEU | CYS | PRO | VAL | GLN | |
10 | GLY | ASN | GLN | GLU | PHE | CYS | GLY | GLN | THR | THR | |
11 | SER | ASN | PRO | THR | ASN | GLN | HIS | GLY | MET | PRO | |
12 | PHE | HIS | PHE | ASP | ILE | CYS | GLU | ASP | THR | GLY | |
13 | GLY | SER | ALA | LYS | PHE | PHE | PRO | SER | GLY | HIS | |
14 | GLY | ALA | LEU | THR | GLY | THR | PHE | THR | GLU | VAL | |
15 | SER | CYS | SER | GLN | TRP | SER | GLY | SER | ASP | GLY | |
16 | GLY | GLN | LEU | TRP | ASN | GLY | ALA | CYS | LEU | SER | |
17 | GLY | GLU | THR | ALA | PRO | ASN | TRP | PRO | SER | THR | |
18 | ALA | CYS | GLY | ASN | LYS | GLY | THR | ALA | PRO | SER |
Samples:
sample_1: PcCel45A, [U-70% 13C; U-95% 15N], 450 uM; D2O, [U-99% 2H], 10%; potassium phosphate 50 mM; sodium azide 0.03%
sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HNCACO | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v4.1.4 - collection, processing
CcpNMR - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Bruker AVANCE III 600 MHz MHz
Related Database Links:
PDB |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts