BMRB Entry 52450
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52450
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Title: Assignment of the Lassa virus glycoprotein 2 transmembrane domain in the postfusion state PubMed: 38916786
Deposition date: 2024-05-10 Original release date: 2024-07-19
Authors: Keating, Patrick; Lee, Jinwoo
Citation: Keating, Patrick; Lee, Jinwoo. "Assignment of the Lassa virus transmembrane domain in the prefusion and postfusion states in detergent micelles" Biomol. NMR Assign. ., .-. (2024).
Assembly members:
entity_1, polymer, 40 residues, Formula weight is not available
Natural source: Common Name: Mammarenavirus lassaense Taxonomy ID: 3052310 Superkingdom: Viruses Kingdom: not available Genus/species: Mammarenavirus lassaense
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24a
Entity Sequences (FASTA):
entity_1: GSGKTPLGLVDLFVFSTSFY
LISIFLHLVKIPTHRHIVGK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 109 |
| 15N chemical shifts | 36 |
| 1H chemical shifts | 36 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Lassa virus transmembrane domain | 1 |
Entities:
Entity 1, Lassa virus transmembrane domain 40 residues - Formula weight is not available
| 1 | GLY | SER | GLY | LYS | THR | PRO | LEU | GLY | LEU | VAL | |
| 2 | ASP | LEU | PHE | VAL | PHE | SER | THR | SER | PHE | TYR | |
| 3 | LEU | ILE | SER | ILE | PHE | LEU | HIS | LEU | VAL | LYS | |
| 4 | ILE | PRO | THR | HIS | ARG | HIS | ILE | VAL | GLY | LYS |
Samples:
sample_1: Lassa virus transmembrane domain, [U-13C; U-15N; U-2H], 400 uM; sodium chloride 100 mM; sodium phosphate 25 mM; LMPG 100 mM
sample_conditions_1: pH: 4.0; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe - processing
NMRbox - data analysis
TOPSPIN - collection
NMRFAM-SPARKY - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts