BMRB Entry 52465

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52465

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Title:
1H, 13C and 15N resonance assignments for the acetyltransferase domain of the kinetoplastid kinetochore protein KKT23 in the presence of coenzyme A
Deposition date:
2024-05-14
Original release date:
2025-03-18
Authors:
Ludzia, Patryk; Nugent, Charlotte; Akiyoshi, Bungo; Redfield, Christina
Citation:

Citation: Ludzia, Patryk; Nugent, Charlotte; Akiyoshi, Bungo; Redfield, Christina. "1 H, 13 C and 15 N resonance assignments for the acetyltransferase domain of the kinetoplastid kinetochore protein KKT23 from Trypanosoma brucei"  Biomol. NMR Assignments ., .-. (2024).

Assembly members:

Assembly members:
entity_1, polymer, 226 residues, 26086.91 Da.
entity_COA, non-polymer, 767.534 Da.

Natural source:

Natural source:   Common Name: Trypanosoma brucei brucei   Taxonomy ID: 5702   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Trypanosoma brucei

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pRSF_Duet-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSQQLTYSQLVLRTAIQDQY SKLSGDGPFPMAFGLVLSEE ERREVIDLYSLQFQYPDQPE LQRLVILPQTHSTRTRRRAK GSYTWYLRSLNTNEMVCAVT IMAHHYETHHFVEVPLFATG VGYKKHGFGRLMNAALLQWC VETGFEFVMISADVKAIPFW SHLGYKTMEKSELTRIVFYY EHNCYKFKGAEVMIRYCRTW PTDGVKEALARVQKVIVSGH VGLMDA

Data sets:
Data typeCount
13C chemical shifts496
15N chemical shifts200
1H chemical shifts233

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1KKT4 125-3481
2Coenzyme A2

Entities:

Entity 1, KKT4 125-348 226 residues - 26086.91 Da.

Residues 1-2 (123G, 124S) represent part of a linker that remained after TEV protease cleavage.

1   GLYSERGLNGLNLEUTHRTYRSERGLNLEU
2   VALLEUARGTHRALAILEGLNASPGLNTYR
3   SERLYSLEUSERGLYASPGLYPROPHEPRO
4   METALAPHEGLYLEUVALLEUSERGLUGLU
5   GLUARGARGGLUVALILEASPLEUTYRSER
6   LEUGLNPHEGLNTYRPROASPGLNPROGLU
7   LEUGLNARGLEUVALILELEUPROGLNTHR
8   HISSERTHRARGTHRARGARGARGALALYS
9   GLYSERTYRTHRTRPTYRLEUARGSERLEU
10   ASNTHRASNGLUMETVALCYSALAVALTHR
11   ILEMETALAHISHISTYRGLUTHRHISHIS
12   PHEVALGLUVALPROLEUPHEALATHRGLY
13   VALGLYTYRLYSLYSHISGLYPHEGLYARG
14   LEUMETASNALAALALEULEUGLNTRPCYS
15   VALGLUTHRGLYPHEGLUPHEVALMETILE
16   SERALAASPVALLYSALAILEPROPHETRP
17   SERHISLEUGLYTYRLYSTHRMETGLULYS
18   SERGLULEUTHRARGILEVALPHETYRTYR
19   GLUHISASNCYSTYRLYSPHELYSGLYALA
20   GLUVALMETILEARGTYRCYSARGTHRTRP
21   PROTHRASPGLYVALLYSGLUALALEUALA
22   ARGVALGLNLYSVALILEVALSERGLYHIS
23   VALGLYLEUMETASPALA

Entity 2, Coenzyme A - C21 H36 N7 O16 P3 S - 767.534 Da.

1   COA

Related Database Links:

TriTrypDB Tb927.10.6600

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks