BMRB Entry 52492
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52492
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Title: Sequence-Specific Backbone assignment of the PP2A-binding domain (aa 62-108) of Eya3
Deposition date: 2024-06-04 Original release date: 2024-07-05
Authors: Godek, Rachel; Peti, Wolfgang; Page, Rebecca
Citation: Godek, Rachel; Padi, Sathish; Peti, Wolfgang; Page, Rebecca. "Cryo-EM structures of PP2A:B55-Eya3 and PP2A:B55-p107 define PP2A:B55 substrate recruitment" .
Assembly members:
entity_1, polymer, 51 residues, 5946.5 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTHMT
Entity Sequences (FASTA):
entity_1: HMRSSNDYTSQMYSAKPYAH
ILSVPVSETAYPGQTQYQTL
QQTQPYAVYEE
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 152 |
15N chemical shifts | 46 |
1H chemical shifts | 46 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Eya3 | 1 |
Entities:
Entity 1, Eya3 51 residues - 5946.5 Da.
Human Eyes absent homolog 3; residues 62-108; Eya 3 starting with RSSNDY
1 | HIS | MET | ARG | SER | SER | ASN | ASP | TYR | THR | SER | ||||
2 | GLN | MET | TYR | SER | ALA | LYS | PRO | TYR | ALA | HIS | ||||
3 | ILE | LEU | SER | VAL | PRO | VAL | SER | GLU | THR | ALA | ||||
4 | TYR | PRO | GLY | GLN | THR | GLN | TYR | GLN | THR | LEU | ||||
5 | GLN | GLN | THR | GLN | PRO | TYR | ALA | VAL | TYR | GLU | ||||
6 | GLU |
Samples:
sample_1: Eya3, [U-99% 15N], 170 uM; Eya3, [U-99% 13C; U-99% 15N], 170 uM; Na2HPO4/NaH2PO4 10 mM; NaCl 200 mM; TCEP 0.5 mM
sample_conditions_1: ionic strength: 200 mM; pH: 6.5; pressure: 1 atm; temperature: 283 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA v1.9 - chemical shift assignment
TOPSPIN v4.1.4 - collection, processing
NMR spectrometers:
- Bruker AVANCE NEO 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts