BMRB Entry 52544
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52544
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Title: Backbone resonance assignemnts of HUWE1 HECT domain N-lobe truncation D4141-4216
Deposition date: 2024-07-10 Original release date: 2024-07-16
Authors: Zhang, Haoran; Li, Yan
Citation: Zhang, Haoran; Li, Yan. "Backbone resonance assignemnts of HUWE1 HECT domain N-lobe truncation D4141-4216" .
Assembly members:
entity_1, polymer, 195 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-28a
Entity Sequences (FASTA):
entity_1: GPGSDFDVKRKYFRQELERL
DEGLRKEDMAVHVRRDHVFE
DSYRELHRKSPEEMKNRLYI
VFEGEEGQDAGGLLREWYMI
ISREMFNPMYALFRTSPGDR
VTYTINPSSHCNPNHLSYFK
FVGRIVAKAVYDNRLLECYF
TRSFYKHILGKSGGGGSGAI
RKQLAAFLEGFYEIIPKRLI
SIFTEQELELLISGL
- assigned_chemical_shifts
- spectral_peak_list
Data type | Count |
13C chemical shifts | 538 |
15N chemical shifts | 179 |
1H chemical shifts | 179 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | HUWE1 HECT domain N-lobe truncation D4141-4216 | 1 |
Entities:
Entity 1, HUWE1 HECT domain N-lobe truncation D4141-4216 195 residues - Formula weight is not available
Original residues 4141-4216 in the full-length HUWE1 HECT domain N-lobe (sequence 'VRYTDMESEDYHFYQGLVYLLENDVSTLGYDLTFSTEVQEFGVCEVRDLKPNGANILVTEENKKEYVHLVCQMRMT') was replaced with a 'GGGGS' linker in this truncated version of HUWE1 HECT domain N-lobe.
1 | GLY | PRO | GLY | SER | ASP | PHE | ASP | VAL | LYS | ARG | ||||
2 | LYS | TYR | PHE | ARG | GLN | GLU | LEU | GLU | ARG | LEU | ||||
3 | ASP | GLU | GLY | LEU | ARG | LYS | GLU | ASP | MET | ALA | ||||
4 | VAL | HIS | VAL | ARG | ARG | ASP | HIS | VAL | PHE | GLU | ||||
5 | ASP | SER | TYR | ARG | GLU | LEU | HIS | ARG | LYS | SER | ||||
6 | PRO | GLU | GLU | MET | LYS | ASN | ARG | LEU | TYR | ILE | ||||
7 | VAL | PHE | GLU | GLY | GLU | GLU | GLY | GLN | ASP | ALA | ||||
8 | GLY | GLY | LEU | LEU | ARG | GLU | TRP | TYR | MET | ILE | ||||
9 | ILE | SER | ARG | GLU | MET | PHE | ASN | PRO | MET | TYR | ||||
10 | ALA | LEU | PHE | ARG | THR | SER | PRO | GLY | ASP | ARG | ||||
11 | VAL | THR | TYR | THR | ILE | ASN | PRO | SER | SER | HIS | ||||
12 | CYS | ASN | PRO | ASN | HIS | LEU | SER | TYR | PHE | LYS | ||||
13 | PHE | VAL | GLY | ARG | ILE | VAL | ALA | LYS | ALA | VAL | ||||
14 | TYR | ASP | ASN | ARG | LEU | LEU | GLU | CYS | TYR | PHE | ||||
15 | THR | ARG | SER | PHE | TYR | LYS | HIS | ILE | LEU | GLY | ||||
16 | LYS | SER | GLY | GLY | GLY | GLY | SER | GLY | ALA | ILE | ||||
17 | ARG | LYS | GLN | LEU | ALA | ALA | PHE | LEU | GLU | GLY | ||||
18 | PHE | TYR | GLU | ILE | ILE | PRO | LYS | ARG | LEU | ILE | ||||
19 | SER | ILE | PHE | THR | GLU | GLN | GLU | LEU | GLU | LEU | ||||
20 | LEU | ILE | SER | GLY | LEU |
Samples:
sample_1: HUWE1 HECT domain N-lobe truncation D4141-4216, [U-99% 13C; U-99% 15N], 600 uM; D2O, [U-100% 2H], 5 % v/v; sodium chloride 200 mM; TRIS 20 mM; TCEP 2 mM
sample_conditions_1: ionic strength: 222.6 mM; pH: 8.0; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HNCACO | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN - collection
NMRPipe - processing
POKY - chemical shift assignment, data analysis, peak picking
NMR spectrometers:
- Bruker AVANCE NEO 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts