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BMRB Entry 52554

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52554

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Title: tau-2N4R

Deposition date: 2024-07-15 Original release date: 2024-07-22

Authors: Theillet, Francois-Xavier; Cherot, Helene

Citation: Theillet, Francois-Xavier; Cherot, Helene. "In-cell residue-resolved NMR of micromolar a-synuclein and tau at 310K"  .

Assembly members:
entity_1, polymer, 442 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-45b(+)

Entity Sequences (FASTA):
entity_1: GMAEPRQEFEVMEDHAGTYG LGDRKDQGGYTMHQDQEGDT DAGLKESPLQTPTEDGSEEP GSETSDAKSTPTAEDVTAPL VDEGAPGKQAAAQPHTEIPE GTTAEEAGIGDTPSLEDEAA GHVTQARMVSKSKDGTGSDD KKAKGADGKTKIATPRGAAP PGQKGQANATRIPAKTPPAP KTPPSSGEPPKSGDRSGYSS PGSPGTPGSRSRTPSLPTPP TREPKKVAVVRTPPKSPSSA KSRLQTAPVPMPDLKNVKSK IGSTENLKHQPGGGKVQIIN KKLDLSNVQSKCGSKDNIKH VPGGGSVQIVYKPVDLSKVT SKCGSLGNIHHKPGGGQVEV KSEKLDFKDRVQSKIGSLDN ITHVPGGGNKKIETHKLTFR ENAKAKTDHGAEIVYKSPVV SGDTSPRHLSNVSSTGSIDM VDSPQLATLADEVSASLAKQ GL

Data sets:
Data typeCount
13C chemical shifts1236
15N chemical shifts392
1H chemical shifts793

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1tau-2N4R1

Entities:

Entity 1, tau-2N4R 442 residues - Formula weight is not available

One supplementary Glycine in N-terminal, remaining from Tev-motif (ENLYFQG) after cleavage by Tev-protease.

1   GLYMETALAGLUPROARGGLNGLUPHEGLU
2   VALMETGLUASPHISALAGLYTHRTYRGLY
3   LEUGLYASPARGLYSASPGLNGLYGLYTYR
4   THRMETHISGLNASPGLNGLUGLYASPTHR
5   ASPALAGLYLEULYSGLUSERPROLEUGLN
6   THRPROTHRGLUASPGLYSERGLUGLUPRO
7   GLYSERGLUTHRSERASPALALYSSERTHR
8   PROTHRALAGLUASPVALTHRALAPROLEU
9   VALASPGLUGLYALAPROGLYLYSGLNALA
10   ALAALAGLNPROHISTHRGLUILEPROGLU
11   GLYTHRTHRALAGLUGLUALAGLYILEGLY
12   ASPTHRPROSERLEUGLUASPGLUALAALA
13   GLYHISVALTHRGLNALAARGMETVALSER
14   LYSSERLYSASPGLYTHRGLYSERASPASP
15   LYSLYSALALYSGLYALAASPGLYLYSTHR
16   LYSILEALATHRPROARGGLYALAALAPRO
17   PROGLYGLNLYSGLYGLNALAASNALATHR
18   ARGILEPROALALYSTHRPROPROALAPRO
19   LYSTHRPROPROSERSERGLYGLUPROPRO
20   LYSSERGLYASPARGSERGLYTYRSERSER
21   PROGLYSERPROGLYTHRPROGLYSERARG
22   SERARGTHRPROSERLEUPROTHRPROPRO
23   THRARGGLUPROLYSLYSVALALAVALVAL
24   ARGTHRPROPROLYSSERPROSERSERALA
25   LYSSERARGLEUGLNTHRALAPROVALPRO
26   METPROASPLEULYSASNVALLYSSERLYS
27   ILEGLYSERTHRGLUASNLEULYSHISGLN
28   PROGLYGLYGLYLYSVALGLNILEILEASN
29   LYSLYSLEUASPLEUSERASNVALGLNSER
30   LYSCYSGLYSERLYSASPASNILELYSHIS
31   VALPROGLYGLYGLYSERVALGLNILEVAL
32   TYRLYSPROVALASPLEUSERLYSVALTHR
33   SERLYSCYSGLYSERLEUGLYASNILEHIS
34   HISLYSPROGLYGLYGLYGLNVALGLUVAL
35   LYSSERGLULYSLEUASPPHELYSASPARG
36   VALGLNSERLYSILEGLYSERLEUASPASN
37   ILETHRHISVALPROGLYGLYGLYASNLYS
38   LYSILEGLUTHRHISLYSLEUTHRPHEARG
39   GLUASNALALYSALALYSTHRASPHISGLY
40   ALAGLUILEVALTYRLYSSERPROVALVAL
41   SERGLYASPTHRSERPROARGHISLEUSER
42   ASNVALSERSERTHRGLYSERILEASPMET
43   VALASPSERPROGLNLEUALATHRLEUALA
44   ASPGLUVALSERALASERLEUALALYSGLN
45   GLYLEU

Samples:

sample_1: tau-2N4R, [U-98% 13C; U-98% 15N], 400 uM; sodium chloride 150 mM; sodium phosphate 20 mM; TCEP 2 mM; DSS 100 uM; sodium azide 0.05 % w/v; protease inhibitors 2 tablet/100mL

sample_conditions_1: ionic strength: 0.190 M; pH: 7.0; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1

Software:

TOPSPIN v4.3 - collection, processing

CcpNMR v2.4 - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE NEO 1200 MHz

Related Database Links:

UNP P10636-8

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts