BMRB Entry 52554
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR52554
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Title: tau-2N4R
Deposition date: 2024-07-15 Original release date: 2024-07-22
Authors: Theillet, Francois-Xavier; Cherot, Helene
Citation: Theillet, Francois-Xavier; Cherot, Helene. "In-cell residue-resolved NMR of micromolar a-synuclein and tau at 310K" .
Assembly members:
entity_1, polymer, 442 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-45b(+)
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 1236 |
15N chemical shifts | 392 |
1H chemical shifts | 793 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | tau-2N4R | 1 |
Entities:
Entity 1, tau-2N4R 442 residues - Formula weight is not available
One supplementary Glycine in N-terminal, remaining from Tev-motif (ENLYFQG) after cleavage by Tev-protease.
1 | GLY | MET | ALA | GLU | PRO | ARG | GLN | GLU | PHE | GLU | ||||
2 | VAL | MET | GLU | ASP | HIS | ALA | GLY | THR | TYR | GLY | ||||
3 | LEU | GLY | ASP | ARG | LYS | ASP | GLN | GLY | GLY | TYR | ||||
4 | THR | MET | HIS | GLN | ASP | GLN | GLU | GLY | ASP | THR | ||||
5 | ASP | ALA | GLY | LEU | LYS | GLU | SER | PRO | LEU | GLN | ||||
6 | THR | PRO | THR | GLU | ASP | GLY | SER | GLU | GLU | PRO | ||||
7 | GLY | SER | GLU | THR | SER | ASP | ALA | LYS | SER | THR | ||||
8 | PRO | THR | ALA | GLU | ASP | VAL | THR | ALA | PRO | LEU | ||||
9 | VAL | ASP | GLU | GLY | ALA | PRO | GLY | LYS | GLN | ALA | ||||
10 | ALA | ALA | GLN | PRO | HIS | THR | GLU | ILE | PRO | GLU | ||||
11 | GLY | THR | THR | ALA | GLU | GLU | ALA | GLY | ILE | GLY | ||||
12 | ASP | THR | PRO | SER | LEU | GLU | ASP | GLU | ALA | ALA | ||||
13 | GLY | HIS | VAL | THR | GLN | ALA | ARG | MET | VAL | SER | ||||
14 | LYS | SER | LYS | ASP | GLY | THR | GLY | SER | ASP | ASP | ||||
15 | LYS | LYS | ALA | LYS | GLY | ALA | ASP | GLY | LYS | THR | ||||
16 | LYS | ILE | ALA | THR | PRO | ARG | GLY | ALA | ALA | PRO | ||||
17 | PRO | GLY | GLN | LYS | GLY | GLN | ALA | ASN | ALA | THR | ||||
18 | ARG | ILE | PRO | ALA | LYS | THR | PRO | PRO | ALA | PRO | ||||
19 | LYS | THR | PRO | PRO | SER | SER | GLY | GLU | PRO | PRO | ||||
20 | LYS | SER | GLY | ASP | ARG | SER | GLY | TYR | SER | SER | ||||
21 | PRO | GLY | SER | PRO | GLY | THR | PRO | GLY | SER | ARG | ||||
22 | SER | ARG | THR | PRO | SER | LEU | PRO | THR | PRO | PRO | ||||
23 | THR | ARG | GLU | PRO | LYS | LYS | VAL | ALA | VAL | VAL | ||||
24 | ARG | THR | PRO | PRO | LYS | SER | PRO | SER | SER | ALA | ||||
25 | LYS | SER | ARG | LEU | GLN | THR | ALA | PRO | VAL | PRO | ||||
26 | MET | PRO | ASP | LEU | LYS | ASN | VAL | LYS | SER | LYS | ||||
27 | ILE | GLY | SER | THR | GLU | ASN | LEU | LYS | HIS | GLN | ||||
28 | PRO | GLY | GLY | GLY | LYS | VAL | GLN | ILE | ILE | ASN | ||||
29 | LYS | LYS | LEU | ASP | LEU | SER | ASN | VAL | GLN | SER | ||||
30 | LYS | CYS | GLY | SER | LYS | ASP | ASN | ILE | LYS | HIS | ||||
31 | VAL | PRO | GLY | GLY | GLY | SER | VAL | GLN | ILE | VAL | ||||
32 | TYR | LYS | PRO | VAL | ASP | LEU | SER | LYS | VAL | THR | ||||
33 | SER | LYS | CYS | GLY | SER | LEU | GLY | ASN | ILE | HIS | ||||
34 | HIS | LYS | PRO | GLY | GLY | GLY | GLN | VAL | GLU | VAL | ||||
35 | LYS | SER | GLU | LYS | LEU | ASP | PHE | LYS | ASP | ARG | ||||
36 | VAL | GLN | SER | LYS | ILE | GLY | SER | LEU | ASP | ASN | ||||
37 | ILE | THR | HIS | VAL | PRO | GLY | GLY | GLY | ASN | LYS | ||||
38 | LYS | ILE | GLU | THR | HIS | LYS | LEU | THR | PHE | ARG | ||||
39 | GLU | ASN | ALA | LYS | ALA | LYS | THR | ASP | HIS | GLY | ||||
40 | ALA | GLU | ILE | VAL | TYR | LYS | SER | PRO | VAL | VAL | ||||
41 | SER | GLY | ASP | THR | SER | PRO | ARG | HIS | LEU | SER | ||||
42 | ASN | VAL | SER | SER | THR | GLY | SER | ILE | ASP | MET | ||||
43 | VAL | ASP | SER | PRO | GLN | LEU | ALA | THR | LEU | ALA | ||||
44 | ASP | GLU | VAL | SER | ALA | SER | LEU | ALA | LYS | GLN | ||||
45 | GLY | LEU |
Samples:
sample_1: tau-2N4R, [U-98% 13C; U-98% 15N], 400 uM; sodium chloride 150 mM; sodium phosphate 20 mM; TCEP 2 mM; DSS 100 uM; sodium azide 0.05 % w/v; protease inhibitors 2 tablet/100mL
sample_conditions_1: ionic strength: 0.190 M; pH: 7.0; pressure: 1 atm; temperature: 283 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
1D 1H | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HNHA | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v4.3 - collection, processing
CcpNMR v2.4 - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE NEO 1200 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts