Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5269
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Citation: Falzone, Christopher; Lecomte, Juliette. "Letter to the Editor: Assignment of the 1H, 13C, and 15N signals of
Synechocystis sp. PCC 6803 methemoglobin" J. Biomol. NMR 23, 71-72 (2002).
PubMed: 12061721
Assembly members:
cyanobacterial hemoglobin, polymer, 124 residues, 14353 Da.
HEM, non-polymer, 616.487 Da.
Natural source: Common Name: Synechocystis sp. PCC 6803 Taxonomy ID: 1148 Superkingdom: Bacteria Kingdom: not available Genus/species: Synechocystis PCC 6803
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
cyanobacterial hemoglobin: MSTLYEKLGGTTAVDLAVDK
FYERVLQDDRIKHFFADVDM
AKQRAHQKAFLTYAFGGTDK
YDGRYMREAHKELVENHGLN
GEHFDAVAEDLLATLKEMGV
PEDLIAEVAAVAGAPAHKRD
VLNQ
Data type | Count |
13C chemical shifts | 494 |
15N chemical shifts | 130 |
1H chemical shifts | 807 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | GlbN | 1 |
2 | Heme | 2 |
Entity 1, GlbN 124 residues - 14353 Da.
1 | MET | SER | THR | LEU | TYR | GLU | LYS | LEU | GLY | GLY | ||||
2 | THR | THR | ALA | VAL | ASP | LEU | ALA | VAL | ASP | LYS | ||||
3 | PHE | TYR | GLU | ARG | VAL | LEU | GLN | ASP | ASP | ARG | ||||
4 | ILE | LYS | HIS | PHE | PHE | ALA | ASP | VAL | ASP | MET | ||||
5 | ALA | LYS | GLN | ARG | ALA | HIS | GLN | LYS | ALA | PHE | ||||
6 | LEU | THR | TYR | ALA | PHE | GLY | GLY | THR | ASP | LYS | ||||
7 | TYR | ASP | GLY | ARG | TYR | MET | ARG | GLU | ALA | HIS | ||||
8 | LYS | GLU | LEU | VAL | GLU | ASN | HIS | GLY | LEU | ASN | ||||
9 | GLY | GLU | HIS | PHE | ASP | ALA | VAL | ALA | GLU | ASP | ||||
10 | LEU | LEU | ALA | THR | LEU | LYS | GLU | MET | GLY | VAL | ||||
11 | PRO | GLU | ASP | LEU | ILE | ALA | GLU | VAL | ALA | ALA | ||||
12 | VAL | ALA | GLY | ALA | PRO | ALA | HIS | LYS | ARG | ASP | ||||
13 | VAL | LEU | ASN | GLN |
Entity 2, Heme - C34 H32 Fe N4 O4 - 616.487 Da.
1 | HEM |
sample_1: cyanobacterial hemoglobin, [U-13C; U-15N], 1 1.5 mM; phosphate 20 mM; H2O 95%; D2O 5%
sample_2: cyanobacterial hemoglobin, [U-15N], 1 1.5 mM; phosphate 20 mM; H2O 95%; D2O 5%
sample_3: cyanobacterial hemoglobin1 2 mM; phosphate 20 mM; H2O 95%; D2O 5%
sample_4: cyanobacterial hemoglobin1 2 mM; phosphate 20 mM; D2O 100%
ex-cond_1: pH: 7.5 na; temperature: 298 K
ex-cond_2: pH*: 7.5 na; temperature: 298 K
ex-cond_3: pH: 6.9 na; temperature: 298 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2QF-COSY | not available | not available | not available |
TOCSY | not available | not available | not available |
NOESY | not available | not available | not available |
1H-15N-NOESY | not available | not available | not available |
1H-15N-TOCSY | not available | not available | not available |
HNCA | not available | not available | not available |
HN(CA)CO | not available | not available | not available |
HNCO | not available | not available | not available |
HCCH-TOCSY | not available | not available | not available |
HCCH-COSY | not available | not available | not available |
CBCA(CO)NH | not available | not available | not available |
CBCANH | not available | not available | not available |
1H-13C HSQC-NOESY | not available | not available | not available |
HCC(CO)NH | not available | not available | not available |
1H-15N HMQC | not available | not available | not available |
1H-13C HMQC | not available | not available | not available |
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