BMRB Entry 5274
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5274
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Title: 1H Chemical shift assignments of Schistocerca gregaria trypsin inhibitor
Deposition date: 2002-02-06 Original release date: 2002-03-29
Authors: Gaspari, Zoltan; Patthy, Andras; Graf, Laszlo; Perczel, Andras
Citation: Gaspari, Zoltan; Patthy, Andras; Graf, Laszlo; Patthy, Andras. "Comparative structure analysis of proteinase inhibitors from the desert locust, Schistocerca gregaria" Eur. J. Biochem. 269, 527-537 (2002).
Assembly members:
Schistocerca gregaria trypsin inhibitor, polymer, 35 residues, Formula weight is not available
Natural source: Common Name: desert locust Taxonomy ID: 7010 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Schistocerca gregaria
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Schistocerca gregaria trypsin inhibitor: EQECTPGQTKKQDCNTCNCT
PTGVWACTRKGCPPH
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 164 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SGTI | 1 |
Entities:
Entity 1, SGTI 35 residues - Formula weight is not available
| 1 | GLU | GLN | GLU | CYS | THR | PRO | GLY | GLN | THR | LYS | ||||
| 2 | LYS | GLN | ASP | CYS | ASN | THR | CYS | ASN | CYS | THR | ||||
| 3 | PRO | THR | GLY | VAL | TRP | ALA | CYS | THR | ARG | LYS | ||||
| 4 | GLY | CYS | PRO | PRO | HIS |
Samples:
sample_1: Schistocerca gregaria trypsin inhibitor 2.4 mM
ex-cond_1: pH: 3.0; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D TOCSY | not available | not available | not available |
| 2D NOESY | not available | not available | not available |
Software:
X-PLOR v3.851 - refinement, structure solution
TRIAD (SYBYL) v6.6, Tripos, Inc. - peak assignment
NMR spectrometers:
- Bruker DRX 500 MHz