BMRB Entry 53018

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53018

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Title:
1H and 15N chemical shift assignments of K63-linked di-ubiquitin with K63R mutation in the distal ubiquitin
Deposition date:
2025-04-09
Original release date:
2025-04-23
Authors:
Kurbah, Iladeiti; T. Lanham, Benjamin; Fushman, David
Citation:

Citation: Kurbah, Iladeiti; Fushman, David. "1H and 15N chemical shift assignments of K63-linked di-ubiquitin with K63R mutation in the distal ubiquitin"  Biomol. NMR Assignments ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 76 residues, 8564.84 Da.
entity_2, polymer, 76 residues, 8592.86 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MQIFVKTLTGKTITLEVEPS DTIENVKAKIQDKEGIPPDQ QRLIFAGKQLEDGRTLSDYN IQKESTLHLVLRLRGG
entity_2: MQIFVKTLTGKTITLEVEPS DTIENVKAKIQDKEGIPPDQ QRLIFAGKQLEDGRTLSDYN IQRESTLHLVLRLRGG

Data sets:
Data typeCount
15N chemical shifts141
1H chemical shifts143

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ubiquitin, chain A1
2ubiquitin, chain B2

Entities:

Entity 1, ubiquitin, chain A 76 residues - 8564.84 Da.

Proximal ubiquitin of K63-linked di-ubiquitin

1   METGLNILEPHEVALLYSTHRLEUTHRGLY
2   LYSTHRILETHRLEUGLUVALGLUPROSER
3   ASPTHRILEGLUASNVALLYSALALYSILE
4   GLNASPLYSGLUGLYILEPROPROASPGLN
5   GLNARGLEUILEPHEALAGLYLYSGLNLEU
6   GLUASPGLYARGTHRLEUSERASPTYRASN
7   ILEGLNLYSGLUSERTHRLEUHISLEUVAL
8   LEUARGLEUARGGLYGLY

Entity 2, ubiquitin, chain B 76 residues - 8592.86 Da.

Distal ubiquitin of K63-linked di-ubiquitin

1   METGLNILEPHEVALLYSTHRLEUTHRGLY
2   LYSTHRILETHRLEUGLUVALGLUPROSER
3   ASPTHRILEGLUASNVALLYSALALYSILE
4   GLNASPLYSGLUGLYILEPROPROASPGLN
5   GLNARGLEUILEPHEALAGLYLYSGLNLEU
6   GLUASPGLYARGTHRLEUSERASPTYRASN
7   ILEGLNARGGLUSERTHRLEUHISLEUVAL
8   LEUARGLEUARGGLYGLY

Related Database Links:

UNP P0CG47 P0CG47

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks