BMRB Entry 5341
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5341
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Title: 1H, 13C, and 15N backbone assignment of an extended N-terminal, DNA-binding domain construct of the replication initiation protein from a geminivirus
Deposition date: 2002-04-09 Original release date: 2002-11-04
Authors: Campos-Olivas, Ramon; Louis, John; Gronenborn, Bruno; Gronenborn, Angela
Citation: Campos-Olivas, Ramon; Louis, John; Clerot, D.; Gronenborn, Bruno; Gronenborn, Angela. "1H, 13C, and 15N assignment of the N-terminal, catalytic domain of the replication initiation protein from the geminivirus TYLCV" J. Biomol. NMR 24, 73-74 (2002).
Assembly members:
N-terminal DNA-binding domain of Rep protein from a geminivirus, polymer, 159 residues, 17922.92 Da.
Natural source: Common Name: Tomato yellow leaf curl virus - Sardinia Taxonomy ID: 123735 Superkingdom: Viruses Kingdom: not available Genus/species: Begomovirus Tomato yellow leaf curl virus - Sardinia
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
N-terminal DNA-binding domain of Rep protein from a geminivirus: MRGSHHHHHHGIPGSGSGDD
DDKMPRSGRFSIKAKNYFLT
YPKCDLTKENALSQITNLQT
PTNKLFIKICRELHENGEPH
LHILIQFEGKYNCTNQRFFD
LVSPTRSAHFHPNIQGAKSS
SDVKSYIDKDGDVLEWGTFQ
IDGRSARGGQQTANDAYAK
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 172 |
13C chemical shifts | 290 |
15N chemical shifts | 156 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | NTD-Rep, H6_rep1-136 | 1 |
Entities:
Entity 1, NTD-Rep, H6_rep1-136 159 residues - 17922.92 Da.
1 | MET | ARG | GLY | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
2 | GLY | ILE | PRO | GLY | SER | GLY | SER | GLY | ASP | ASP | ||||
3 | ASP | ASP | LYS | MET | PRO | ARG | SER | GLY | ARG | PHE | ||||
4 | SER | ILE | LYS | ALA | LYS | ASN | TYR | PHE | LEU | THR | ||||
5 | TYR | PRO | LYS | CYS | ASP | LEU | THR | LYS | GLU | ASN | ||||
6 | ALA | LEU | SER | GLN | ILE | THR | ASN | LEU | GLN | THR | ||||
7 | PRO | THR | ASN | LYS | LEU | PHE | ILE | LYS | ILE | CYS | ||||
8 | ARG | GLU | LEU | HIS | GLU | ASN | GLY | GLU | PRO | HIS | ||||
9 | LEU | HIS | ILE | LEU | ILE | GLN | PHE | GLU | GLY | LYS | ||||
10 | TYR | ASN | CYS | THR | ASN | GLN | ARG | PHE | PHE | ASP | ||||
11 | LEU | VAL | SER | PRO | THR | ARG | SER | ALA | HIS | PHE | ||||
12 | HIS | PRO | ASN | ILE | GLN | GLY | ALA | LYS | SER | SER | ||||
13 | SER | ASP | VAL | LYS | SER | TYR | ILE | ASP | LYS | ASP | ||||
14 | GLY | ASP | VAL | LEU | GLU | TRP | GLY | THR | PHE | GLN | ||||
15 | ILE | ASP | GLY | ARG | SER | ALA | ARG | GLY | GLY | GLN | ||||
16 | GLN | THR | ALA | ASN | ASP | ALA | TYR | ALA | LYS |
Samples:
sample1: N-terminal DNA-binding domain of Rep protein from a geminivirus, [10%-13C; U-99% 15N], 0.6 0.8 mM; sodium phosphate 20 mM; NaCl 300 mM; DTT 1 mM; D2O 8%; H2O 92%
sample2: N-terminal DNA-binding domain of Rep protein from a geminivirus, [U-13C; U-99% 15N], 0.6 0.8 mM; sodium phosphate 20 mM; NaCl 300 mM; DTT 1 mM; D2O 8%; H2O 92%
sample3: N-terminal DNA-binding domain of Rep protein from a geminivirus, [U-10% 13C; U-99% 15N], 0.6 0.8 mM; sodium phosphate 20 mM; NaCl 300 mM; DTT 1 mM; D2O 100%
sample4: N-terminal DNA-binding domain of Rep protein from a geminivirus, [U-13C; U-99% 15N], 0.6 0.8 mM; sodium phosphate 20 mM; NaCl 300 mM; DTT 1 mM; D2O 100%
cond_H2O: pH: 6.6; temperature: 298 K; ionic strength: 1.0 M
cond_D2O: pH*: 6.6; temperature: 298 K; ionic strength: 1.0 M
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H NOESY | not available | not available | not available |
2D 1H-1H TOCSY | not available | not available | not available |
2D 1H-15N HSQC | not available | not available | not available |
2D 1H-13C HSQC/HMQC | not available | not available | not available |
3D HNCO | not available | not available | not available |
3D HNCA | not available | not available | not available |
3D HNCOCA | not available | not available | not available |
3D HNCACB | not available | not available | not available |
3D CC(CO)NH | not available | not available | not available |
3D CBCA(CO)NH | not available | not available | not available |
3D 15N-edited NOESY | not available | not available | not available |
4D 13C,13C NOESY | not available | not available | not available |
Software:
nmrpipe - processing
nmrview v4.1.1 - assignment
NMR spectrometers:
- Bruker DMX 500 MHz
- Bruker DMX 600 MHz
- Bruker DRX 600 MHz
- Bruker DMX 750 MHz
- Bruker DRX 800 MHz
Related Database Links:
BMRB | 5297 |
PDB | |
EMBL | CAA43466 |
GB | ACE02956 ACE02974 ADF43040 AFQ00071 |
REF | NP_620741 |
SP | P27260 |
AlphaFold | P27260 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts