BMRB Entry 5711
Chem Shift validation: LACS
BMRB Entry DOI: doi:10.13018/BMR5711
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Title: Solution Structure of HP0371, Biotin Carboxyl Carrier protein from Helocobacter pylori
Deposition date: 2003-03-01 Original release date: 2009-03-17
Authors: Jung, Jinwon; Lee, Weontae
Citation: Jung, Jinwon; Lee, Chul-Jin; Jeon, Young Ho; Cheong, Chaejoon; Lee, Weontae. "Solution structure and backbone dynamics of the biotinylation domain of Helicobacter pylori biotin-carboxyl carrier protein" B. Korean Chem. Soc. 29, 347-351 (2008).
Assembly members:
Biotinoyl domain of HP0371, polymer, 78 residues, Formula weight is not available
Natural source: Common Name: Helicobater pylori Taxonomy ID: not available Superkingdom: Eubacteria Kingdom: not available Genus/species: Helicobater pylori
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-15b
Entity Sequences (FASTA):
Biotinoyl domain of HP0371: GSVLSPMVGTFYHAPSPGAE
PYVKAGDTLKKGQIVGIVEA
MKIMNEIEVEYPCKVVSVEV
GDAQPVEYGTKLIKVEKL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 211 |
| 15N chemical shifts | 68 |
| 1H chemical shifts | 440 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Biotinoyl domain of HP0371 | 1 |
Entities:
Entity 1, Biotinoyl domain of HP0371 78 residues - Formula weight is not available
| 1 | GLY | SER | VAL | LEU | SER | PRO | MET | VAL | GLY | THR | ||||
| 2 | PHE | TYR | HIS | ALA | PRO | SER | PRO | GLY | ALA | GLU | ||||
| 3 | PRO | TYR | VAL | LYS | ALA | GLY | ASP | THR | LEU | LYS | ||||
| 4 | LYS | GLY | GLN | ILE | VAL | GLY | ILE | VAL | GLU | ALA | ||||
| 5 | MET | LYS | ILE | MET | ASN | GLU | ILE | GLU | VAL | GLU | ||||
| 6 | TYR | PRO | CYS | LYS | VAL | VAL | SER | VAL | GLU | VAL | ||||
| 7 | GLY | ASP | ALA | GLN | PRO | VAL | GLU | TYR | GLY | THR | ||||
| 8 | LYS | LEU | ILE | LYS | VAL | GLU | LYS | LEU |
Samples:
sample_15N: Biotinoyl domain of HP0371, [U-95% 15N], 2 mM; sodium phosphate buffer 50 mM; NaCl 150 mM; DTT 1 mM
sample_13C_15N: Biotinoyl domain of HP0371, [U-95% 13C; U-95% 15N], 0.7 mM; sodium phosphate buffer 50 mM; NaCl 150 mM; DTT 1 mM
sample_13C: Biotinoyl domain of HP0371, [U-95% 13C], 0.4 mM; sodium phosphate buffer 50 mM; NaCl 150 mM; DTT 1 mM
Ex-cond_1: ionic strength: 0.3 M; pH: 7.0 na; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| 1H-13C CT-HSQC | not available | not available | not available |
| 1H-15C TOCSY | not available | not available | not available |
| 1H-15C NOESY | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| 1H-13C NOESY | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HNHA | not available | not available | not available |
Software:
NMRPipe v2.1 - processing of data
SPARKY v3.106 - peak assignment, peak picking
AutoAssign v1.7 - automated backbond assignment
CYANA v1.0.6 - structure calculation with automated NOESY assignment
NMR spectrometers:
- Bruker DRX 500 MHz
- Varian INOVA-UNITY-Plus 500 MHz
Related Database Links:
| DBJ | BAJ55623 BAJ56401 BAJ58603 BAJ60120 BAM96952 |
| EMBL | CAX29567 CBI65976 |
| GB | AAD07435 ABF85088 ACD48521 ACI27779 ACJ08202 |
| REF | NP_207169 WP_001053746 WP_001053747 WP_001053748 WP_001053749 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts