BMRB Entry 5870
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5870
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Title: Structural Studies of Cu(I)-Bleomycin PubMed: 15015041
Deposition date: 2003-07-16 Original release date: 2004-10-25
Authors: Lehmann, T.
Citation: Lehmann, T.. "Structural Study of Cu(I)-Bleomycin" J. Biol. Inorg. Chem. 9, 323-334 (2004).
Assembly members:
BLEOMYCIN A2, non-polymer, 1416.560 Da.
CU1, non-polymer, 63.546 Da.
Natural source: Common Name: Streptomyces verticillus Taxonomy ID: 29309 Superkingdom: Bacteria Kingdom: not available Genus/species: Streptomyces verticillus
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 70 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | BLEOMYCIN A2 | 1 |
| 2 | COPPER (I) ION | 2 |
Entities:
Entity 1, BLEOMYCIN A2 1 residues - C55 H85 N17 O21 S3 - 1416.560 Da.
| 1 | BLM |
Entity 2, COPPER (I) ION - Cu - 63.546 Da.
| 1 | CU1 |
Samples:
sample_1: BLEOMYCIN A2 5 mM; COPPER (I) ION 5 mM; H2O 90%; D2O 10%
sample_2: BLEOMYCIN A2 5 mM; COPPER (I) ION 5 mM; D2O 100%
sample_cond_1: ionic strength: 5 mM; pH: 8.1; pressure: 1 atm; temperature: 278 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | sample_cond_1 |
Software:
xwinnmr v2.5 - collection
NMRPipe v2002 - processing
DISCOVER v2003 - refinement, structure solution
NMR spectrometers:
- Bruker DRX 500 MHz