BMRB Entry 5877
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5877
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Thiriot, D.; Nevzorov, A.; Zagyanskiy, L.; Wu, C.; Opella, S.. "Structure of the coat protein in Pf1 bacteriophage determined by
solid-state NMR spectroscopy." J. Mol. Biol. 341, 869-879 (2004).
PubMed: 15288792
Assembly members:
Pf1 phage major coat protein, polymer, 46 residues, Formula weight is not available
Natural source: Common Name: Pseudomonas phage Pf1 Taxonomy ID: 10871 Superkingdom: Viruses Kingdom: not available Genus/species: Inovirus Pseudomonas phage Pf1
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
Pf1 phage major coat protein: GVIDTSAVESAITDGQGDMK
AIGGYIVGALVILAVAGLIY
SMLRKA
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 15N chemical shifts | 43 |
| coupling constants | 43 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Pf1 major coat protein | 1 |
Entities:
Entity 1, Pf1 major coat protein 46 residues - Formula weight is not available
| 1 | GLY | VAL | ILE | ASP | THR | SER | ALA | VAL | GLU | SER | ||||
| 2 | ALA | ILE | THR | ASP | GLY | GLN | GLY | ASP | MET | LYS | ||||
| 3 | ALA | ILE | GLY | GLY | TYR | ILE | VAL | GLY | ALA | LEU | ||||
| 4 | VAL | ILE | LEU | ALA | VAL | ALA | GLY | LEU | ILE | TYR | ||||
| 5 | SER | MET | LEU | ARG | LYS | ALA |
Samples:
sample_1: Pf1 phage major coat protein, [U-15N], 50 mg/mL; sodium borate buffer 5 mM; H2O M
sample_cond_1: pH: 8 na; temperature: 303 K; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| PISEMA | not available | not available | not available |
| Magic Sammy | not available | not available | not available |
Software:
FELIX v2000.1 - processing
XWINNMR, MacNMR, NTNMR - collection
MATLAB script v6.5 - structure solution
MATLAB scripts v6.5 - refinement
SCWRL v2.95 - Blue Horizon modified - refinement
SCWRL v2.95 - refinement
NMR spectrometers:
- Bruker AVANCE 750 MHz
- Homebuilt Apollo 550 MHz