BMRB Entry 6050
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6050
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Title: Solution structure of Sso7d mutant (K12L), NMR, 4 structures PubMed: 17340638
Deposition date: 2003-12-16 Original release date: 2007-11-05
Authors: Recca, T.; Consonni, R.; Alberti, E.; Arosio, I.; Zetta, L.
Citation: Consonni, R.; Arosio, I.; Recca, T.; Fusi, P.; Zetta, L.. "Structural determinants responsible for the thermostability of Sso7d and its single point mutants" Proteins 67, 766-775 (2007).
Assembly members:
SSO7D, polymer, 62 residues, Formula weight is not available
Natural source: Common Name: Sulfolobus solfataricus Taxonomy ID: 2287 Superkingdom: Archaea Kingdom: not available Genus/species: Sulfolobus solfataricus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
SSO7D: ATVKFKYKGEELQVDISKIK
KVWRVGKMISFTYDEGGGKT
GRGAVSEKDAPKKLLQMLEK
QK
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 446 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Endoribounuclease SSO7D | 1 |
Entities:
Entity 1, Endoribounuclease SSO7D 62 residues - Formula weight is not available
1 | ALA | THR | VAL | LYS | PHE | LYS | TYR | LYS | GLY | GLU | ||||
2 | GLU | LEU | GLN | VAL | ASP | ILE | SER | LYS | ILE | LYS | ||||
3 | LYS | VAL | TRP | ARG | VAL | GLY | LYS | MET | ILE | SER | ||||
4 | PHE | THR | TYR | ASP | GLU | GLY | GLY | GLY | LYS | THR | ||||
5 | GLY | ARG | GLY | ALA | VAL | SER | GLU | LYS | ASP | ALA | ||||
6 | PRO | LYS | LYS | LEU | LEU | GLN | MET | LEU | GLU | LYS | ||||
7 | GLN | LYS |
Samples:
sample_1: SSO7D 2 mM; D2O 10%; H2O 90%
sample_2: SSO7D 2 mM; D2O 100%; H2O 90%
sample_cond_1: pH: 4.5; pressure: 1 atm; temperature: 300 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
DQF-COSY | not available | not available | not available |
2D TOCSY | not available | not available | not available |
2D NOESY | not available | not available | not available |
Software:
FELIX v98.0 - data analysis
DISCOVER v3.0 - refinement, structure solution
xwinnmr v2.6 - collection
NMR spectrometers:
- Bruker DMX 500 MHz
Related Database Links:
PDB |