BMRB Entry 6131
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR6131
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Solution structure of oxidized bovine microsomal cytochrome B5 Mutant V45H
Deposition date: 2004-03-04 Original release date: 2005-03-03
Authors: Wu, H.; Zhang, Q.
Citation: Wu, H.; Zhang, Q.. "The comparative study on the solution structures of the oxidized boving microsomal cytochrome b5 and mutant V45H" Protein Sci. 13, 2161-2169 (2004).
Assembly members:
Cytochrome b5 Mutant V45H, polymer, 82 residues, Formula weight is not available
HEM, non-polymer, 616.487 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Cytochrome b5 Mutant V45H: AVKYYTLEEIQKHNNSKSTW
LILHYKVYDLTKFLEEHPGG
EEHLREQAGGDATENFEDVG
HSTDARELSKTFIIGELHPD
DR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 462 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Cytochrome b5 | 1 |
| 2 | HEM_ox | 2 |
Entities:
Entity 1, Cytochrome b5 82 residues - Formula weight is not available
| 1 | ALA | VAL | LYS | TYR | TYR | THR | LEU | GLU | GLU | ILE | ||||
| 2 | GLN | LYS | HIS | ASN | ASN | SER | LYS | SER | THR | TRP | ||||
| 3 | LEU | ILE | LEU | HIS | TYR | LYS | VAL | TYR | ASP | LEU | ||||
| 4 | THR | LYS | PHE | LEU | GLU | GLU | HIS | PRO | GLY | GLY | ||||
| 5 | GLU | GLU | HIS | LEU | ARG | GLU | GLN | ALA | GLY | GLY | ||||
| 6 | ASP | ALA | THR | GLU | ASN | PHE | GLU | ASP | VAL | GLY | ||||
| 7 | HIS | SER | THR | ASP | ALA | ARG | GLU | LEU | SER | LYS | ||||
| 8 | THR | PHE | ILE | ILE | GLY | GLU | LEU | HIS | PRO | ASP | ||||
| 9 | ASP | ARG |
Entity 2, HEM_ox - C34 H32 Fe N4 O4 - 616.487 Da.
| 1 | HEM |
Samples:
sample_1: Cytochrome b5 Mutant V45H 3.0 mM; PROTOPORPHYRIN IX CONTAINING FE 3.0 mM; H2O 90%; D2O 10%; Phosphate buffer 25 mM
sample_2: Cytochrome b5 Mutant V45H 3.0 mM; PROTOPORPHYRIN IX CONTAINING FE 3.0 mM; D2O 100%; Phosphate buffer 25 mM
sample_cond_1: pH: 7.00; temperature: 293 K; ionic strength: 25 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | sample_cond_1 |
| 2D TOCSY | not available | not available | sample_cond_1 |
| DQF-COSY | not available | not available | sample_cond_1 |
Software:
DYANA v1.5 - structure solution
VNMR v6.1B - collection
XEASY - data analysis
AMBER v6.0 - refinement
NMR spectrometers:
- UNITY Varian 800 MHz